Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PYGL (phospho S15) antibody [EPR20881-72] - BSA and Azide free (ab256553)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20881-72] to PYGL (phospho S15) - BSA and Azide free
  • Suitable for: IP, Dot blot, WB, IHC-Fr, IHC-P
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-PYGL (phospho S15) antibody [EPR20881-72] - BSA and Azide free
    See all PYGL primary antibodies

  • Description

    Rabbit monoclonal [EPR20881-72] to PYGL (phospho S15) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, Dot blot, WB, IHC-Fr, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse and rat liver tissue. IHC-Fr: Mouse and rat liver tissue. IP: Mouse liver tissue.

  • General notes

    ab256553 is the carrier-free version of ab227043. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Dot Blot - Anti-PYGL (phospho S15) antibody [EPR20881-72] - BSA and Azide free (ab256553)
    Dot Blot - Anti-PYGL (phospho S15) antibody [EPR20881-72] - BSA and Azide free (ab256553)

    Dot blot analysis of PYGL (phospho S15) labeled with ab227043 at 1/1000 dilution.

    Lane 1: Mouse PYGL (phospho S15) peptide (aa9-19).
    Lane 2: Mouse PYGL (phospho S15) peptide (aa10-20).
    Lane 3: Mouse PYGL non-phospho peptide (aa9-20).

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227043).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYGL (phospho S15) antibody [EPR20881-72] - BSA and Azide free (ab256553)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYGL (phospho S15) antibody [EPR20881-72] - BSA and Azide free (ab256553)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling PYGL (phospho S15) with ab227043 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse liver without AP treatment (Panel A), no staining was observed after AP treatment (Panel B).The section was incubated with ab227043 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227043).

  • Immunohistochemistry (Frozen sections) - Anti-PYGL (phospho S15) antibody [EPR20881-72] - BSA and Azide free (ab256553)
    Immunohistochemistry (Frozen sections) - Anti-PYGL (phospho S15) antibody [EPR20881-72] - BSA and Azide free (ab256553)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling PYGL (phospho S15) with ab227043 at 100 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1000 dilution (Green). Cytoplasmic staining on mouse liver, and no staining upon alkaline phosphatase (AP) treatment for 1 h. is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227043).

  • Immunoprecipitation - Anti-PYGL (phospho S15) antibody [EPR20881-72] - BSA and Azide free (ab256553)
    Immunoprecipitation - Anti-PYGL (phospho S15) antibody [EPR20881-72] - BSA and Azide free (ab256553)

    PYGL (phospho S15) was immunoprecipitated from 0.35 mg mouse liver lysate with ab227043 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab227043 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse liver lysate 10ug

    Lane 2: ab227043 IP in mouse liver lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab227043 in mouse liver lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227043).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYGL (phospho S15) antibody [EPR20881-72] - BSA and Azide free (ab256553)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYGL (phospho S15) antibody [EPR20881-72] - BSA and Azide free (ab256553)

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling PYGL (phospho S15) with ab227043 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat liver without AP treatment (Panel A), no staining was observed after AP treatment (Panel B).The section was incubated with ab227043 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227043).

  • Immunohistochemistry (Frozen sections) - Anti-PYGL (phospho S15) antibody [EPR20881-72] - BSA and Azide free (ab256553)
    Immunohistochemistry (Frozen sections) - Anti-PYGL (phospho S15) antibody [EPR20881-72] - BSA and Azide free (ab256553)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat liver tissue labeling PYGL (phospho S15) with ab227043 at 100 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1000 dilution (Green). Cytoplasmic staining on rat liver, and no staining upon alkaline phosphatase (AP) treatment for 1 h. is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondaryat 1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227043).

  • Anti-PYGL (phospho S15) antibody [EPR20881-72] - BSA and Azide free (ab256553)
    Anti-PYGL (phospho S15) antibody [EPR20881-72] - BSA and Azide free (ab256553)

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