Human HTT (Huntingtin) knockout HeLa cell lysate (ab256946)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab262819 - Human HTT knockout HeLa cell lysate 1 x 100µg ab255929 - Human wild-type HeLa cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8,12 CSF1PO: 9, 10
Images
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Western blot - Human HTT (Huntingtin) knockout HeLa cell lysate (ab256946)
Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: HTT knockout HeLa cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab109115 observed at 348 kDa. Red - loading control ab7291 observed at 50 kDa.
ab109115 Anti-Huntingtin antibody [EPR5526] was shown to specifically react with Huntingtin in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265976 (knockout cell lysate ab256946) was used. Wild-type and Huntingtin knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109115 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 10000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Western blot - Human HTT (Huntingtin) knockout HeLa cell lysate (ab256946)Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: HTT knockout HeLa cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab45169 observed at 348 kDa. Red - loading control ab7291 observed at 50 kDa.
ab45169 Anti-Huntingtin antibody [EP867Y] was shown to specifically react with Huntingtin in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265976 (knockout cell lysate ab256946) was used. Wild-type and Huntingtin knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab45169 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 10000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human HTT knockout HeLa cell lysate (ab256946)Homozygous: 1 bp deletion in exon21