Anti-GAPDH antibody - Loading Control (ab37168)
Key features and details
- Rabbit polyclonal to GAPDH - Loading Control
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GAPDH antibody - Loading Control
See all GAPDH primary antibodies -
Description
Rabbit polyclonal to GAPDH - Loading Control -
Host species
Rabbit -
Specificity
Can be used for the detection of GAPDH. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human GAPDH aa 60-110 (N terminal). Synthetic peptide, corresponding to 16 N terminal amino acids of Human GAPDH
Database link: P04406
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
GAPDH Antibody is affinity chromatography purified via peptide column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GAPDH antibody - Loading Control (ab37168)Lane 1 : Anti-GAPDH antibody - Loading Control (ab37168) at 0.125 µg/ml
Lane 2 : Anti-GAPDH antibody - Loading Control (ab37168) at 0.25 µg/ml
Lane 3 : Anti-GAPDH antibody - Loading Control (ab37168) at 0.5 µg/ml
All lanes : HeLa cell lysate with GAPDH peptide
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : anti-rabbit IgG HRP at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (ab37168)ICC/IF image of ab37168 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37168, 1mg/ml) overnight at +4øC. The secondary antibody (green) was anti-rabbit Alexa Fluor® 488 (ab150077) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.
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Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (ab37168)ICC/IF image of ab37168 stained Hela cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37168, 1µg/ml) overnight at +4°C. The secondary antibody (green) was anti-Rabbit DyLight® 488 pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (ab37168)Immunofluorescence of GAPDH in HeLa cells using ab37168 at 10 ug/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody - Loading Control (ab37168)ab37168 at 10µg/ml staining GAPDH in human liver tissue by IHC
