HRP Anti-GAPDH antibody - Loading Control (ab9385)
Key features and details
- HRP Rabbit polyclonal to GAPDH - Loading Control
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG
Overview
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Product name
HRP Anti-GAPDH antibody - Loading Control
See all GAPDH primary antibodies -
Description
HRP Rabbit polyclonal to GAPDH - Loading Control -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Saccharomyces cerevisiae, Xenopus laevis
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Immunogen
Full length native protein (purified) corresponding to Human GAPDH.
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Positive control
- This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; A431; Hek293.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
This antibody is the HRP conjugated version of ab9485, for more convenient use as a loading control antibody. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-GAPDH antibody - Loading Control (ab9385)All lanes : HRP Anti-GAPDH antibody - Loading Control (ab9385) at 1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteLane 1 - 4 : GAPDH antibody - Loading Control (ab9385) at 1 ug/ml
Lane 1 : HeLa cell lysate at 20 ug
Lane 2 : A431 cell lysate at 20 ug
Lane 3 : Jurkat cell lysate at 20 ug
Lane 4 : 293 cell lysate at 20 ugPerformed under reducing conditions.
Exposure time : 1 minute
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Western blot - HRP Anti-GAPDH antibody - Loading Control (ab9385)Lanes 1-4 : HRP Anti-GAPDH antibody - Loading Control (ab9385) at 1/5000 dilution ((Blocked in 5% BSA))
Lanes 5-8 : HRP Anti-GAPDH antibody - Loading Control (ab9385) at 1/5000 dilution ((Blocked in 5% MILK))
Lanes 1 & 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252)
Lanes 2 & 6 : Jurkat (Human) Whole Cell Lysate (ab52254)
Lanes 3 & 7 :A-431 whole cell lysate (ab7909)
Lanes 4 & 8 :HEK-293 whole cell lysate (ab7902)
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
The membrane 1-4 was blocked in 5% BSA (1 hour). The membrane 5-8 was blocked in 5% MILK (1 hour). Abcam routinely uses 5% BSA to block, however following recent customer feedback our labs investigated the effect of 5% milk blocking. We can now confirm that milk is not a suitable blocking agent for this antibody and significantly decreases the signal on the membrane.
