Anti-Huntingtin antibody (ab155942)
Key features and details
- Rabbit polyclonal to Huntingtin
- Suitable for: IHC-Fr, WB, ELISA, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Huntingtin antibody
See all Huntingtin primary antibodies -
Description
Rabbit polyclonal to Huntingtin -
Host species
Rabbit -
Specificity
ab155942 recognizes the 552 cleaved fragment without detecting the full-length form. -
Tested applications
Suitable for: IHC-Fr, WB, ELISA, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human Huntingtin aa 544-552 conjugated to Keyhole Limpet Haemocyanin (KLH) (Cysteine residue).
Sequence:SDPAMDLND
Database link: P42858 -
Positive control
- Huntingtin protein and protein fragments; 293T cells; Mouse cortex tissue; Endogenous Huntingtin lysates, with or without caspase activity.
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General notes
Human HTT caspase cleavage sites generate fragment-specific forms of the protein. Caspase-3/7 has been shown to generate cleavage sites at animo acids 513 and 552. Caspase-2 cleaves at amino acid 552 and caspase-6 at amino acid 586.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Primary antibody notes
Human HTT caspase cleavage sites generate fragment-specific forms of the protein. Caspase-3/7 has been shown to generate cleavage sites at animo acids 513 and 552. Caspase-2 cleaves at amino acid 552 and caspase-6 at amino acid 586. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Huntingtin antibody (ab155942) at 1/500 dilution
Lane 1 : -
Lane 2 : +C2
Lane 3 : +C3
Lane 4 : +C6
Lane 5 : +C7
Lane 6 : Huntingtin23Q(1-513)
Lane 7 : Huntingtin23Q(1-536)
Lane 8 : Huntingtin23Q(1-552)
Lane 9 : Huntingtin23Q(1-586)
Lysates/proteins at 20 µg per lane.
Predicted band size: 348 kDa
Additional bands at: 62 kDa (possible cleavage fragment)
Lanes 1-5 are 293Tcells expressing the full length Htt protein and treated with the various caspases. Lanes 6-9 are 293T overexpressing the different Htt fragments to demonstrate the antibody’s specificity to that neo-epitope. Probed with N-terminal pan-Huntingtin antibody or (bottom panel) neoepitope specific rabbit polyclonal antibody. -
Sandwich Elisa analysis of 293T cells (overexpressing Huntingtin neo epitope 552), labeling Huntingtin protein (0, 0.5,1,1.5 and 2µg) with ab155942 at 1/20 dilution.
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Immunofluorescent analysis of 293T cells (transfected with Huntingtin23Q and Huntingtin148Q stop constructs ending in amino acid 552), labeling Huntingtin ab155942 at 1/50 dilution.
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Immunohistochemistry analysis of Mouse cortex tissue samples, labeling Huntingtin with ab155942 at 1/50 dilution (green). Blue: Tissue counterstained with nuclei stain.