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Neuroscience Neurology process Neurodegenerative disease Huntington's disease

Anti-Huntingtin antibody (ab155942)

Anti-Huntingtin antibody (ab155942)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Huntingtin
  • Suitable for: IHC-Fr, WB, ELISA, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Huntingtin antibody
    See all Huntingtin primary antibodies
  • Description

    Rabbit polyclonal to Huntingtin
  • Host species

    Rabbit
  • Specificity

    ab155942 recognizes the 552 cleaved fragment without detecting the full-length form.
  • Tested applications

    Suitable for: IHC-Fr, WB, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Huntingtin aa 544-552 conjugated to Keyhole Limpet Haemocyanin (KLH) (Cysteine residue).
    Sequence:

    SDPAMDLND


    Database link: P42858
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Huntingtin protein and protein fragments; 293T cells; Mouse cortex tissue; Endogenous Huntingtin lysates, with or without caspase activity.
  • General notes

    Human HTT caspase cleavage sites generate fragment-specific forms of the protein. Caspase-3/7 has been shown to generate cleavage sites at animo acids 513 and 552. Caspase-2 cleaves at amino acid 552 and caspase-6 at amino acid 586.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Primary antibody notes

    Human HTT caspase cleavage sites generate fragment-specific forms of the protein. Caspase-3/7 has been shown to generate cleavage sites at animo acids 513 and 552. Caspase-2 cleaves at amino acid 552 and caspase-6 at amino acid 586.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Huntington's disease

Images

  • Western blot - Anti-Huntingtin antibody (ab155942)
    Western blot - Anti-Huntingtin antibody (ab155942)
    All lanes : Anti-Huntingtin antibody (ab155942) at 1/500 dilution

    Lane 1 : -
    Lane 2 : +C2
    Lane 3 : +C3
    Lane 4 : +C6
    Lane 5 : +C7
    Lane 6 : Huntingtin23Q(1-513)
    Lane 7 : Huntingtin23Q(1-536)
    Lane 8 : Huntingtin23Q(1-552)
    Lane 9 : Huntingtin23Q(1-586)

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 348 kDa
    Additional bands at: 62 kDa (possible cleavage fragment)



    Lanes 1-5 are 293Tcells expressing the full length Htt protein and treated with the various caspases. Lanes 6-9 are 293T overexpressing the different Htt fragments to demonstrate the antibody’s specificity to that neo-epitope. Probed with N-terminal pan-Huntingtin antibody or (bottom panel) neoepitope specific rabbit polyclonal antibody.
  • ELISA - Anti-Huntingtin antibody (ab155942)
    ELISA - Anti-Huntingtin antibody (ab155942)
    Sandwich Elisa analysis of 293T cells (overexpressing Huntingtin neo epitope 552), labeling Huntingtin protein (0, 0.5,1,1.5 and 2&microg) with ab155942 at 1/20 dilution.
  • Immunocytochemistry/ Immunofluorescence - Anti-Huntingtin antibody (ab155942)
    Immunocytochemistry/ Immunofluorescence - Anti-Huntingtin antibody (ab155942)
    Immunofluorescent analysis of 293T cells (transfected with Huntingtin23Q and Huntingtin148Q stop constructs ending in amino acid 552), labeling Huntingtin ab155942 at 1/50 dilution.
  • Immunohistochemistry (Frozen sections) - Anti-Huntingtin antibody (ab155942)
    Immunohistochemistry (Frozen sections) - Anti-Huntingtin antibody (ab155942)
    Immunohistochemistry analysis of Mouse cortex tissue samples, labeling Huntingtin with ab155942 at 1/50 dilution (green). Blue: Tissue counterstained with nuclei stain.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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