Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Pescadillo antibody [8E9] (ab252849)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [8E9] to Pescadillo
  • Suitable for: WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Pescadillo antibody [8E9]
    See all Pescadillo primary antibodies

  • Description

    Rat monoclonal [8E9] to Pescadillo

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    IF
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HEK-293, HCT116, Jurkat and RAW264.7 whole cell lysate. ICC/IF: HeLa and HEK-293 cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunofluorescence - Anti-Pescadillo antibody [8E9] (ab252849)
    Immunofluorescence - Anti-Pescadillo antibody [8E9] (ab252849)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed,0.1% Triton X-100 permeabilized HEK-293 (human embryonic kidney epithelial cell) labeling Pescadillo with ab252849 at 0.978µg/ml, followed by Goat Anti-Mouse IgG (Alexa Fluor® 488) (ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing nucleolar staining on HEK-293 cell line. The nuclear counterstain is DAPI (blue).

    Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red).

    The negative controls are as follows:

    -ve control 1: ab252849 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution.

    -ve control 2: ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution.

  • Immunofluorescence - Anti-Pescadillo antibody [8E9] (ab252849)
    Immunofluorescence - Anti-Pescadillo antibody [8E9] (ab252849)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed,0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) labeling Pescadillo with ab252849 at 0.978µg/ml, followed by Goat Anti-Mouse IgG (Alexa Fluor® 488) (ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing nucleolar staining on HeLa cell line. The nuclear counterstain is DAPI (blue).

    Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red).

    The negative controls are as follows:

    -ve control 1: ab252849 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution.

    -ve control 2: ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution.

  • Western blot - Anti-Pescadillo antibody [8E9] (ab252849)
    Western blot - Anti-Pescadillo antibody [8E9] (ab252849)
    All lanes : Anti-Pescadillo antibody [8E9] (ab252849) at 0.489 µg/ml

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate
    Lane 3 : HCT116 (human colorectal carcinoma epithelial cell), whole cell lysate
    Lane 4 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
    Lane 5 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/100000 dilution

    Predicted band size: 68 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-4: 1 sec; Lane 5: 5 secs.

  • Anti-Pescadillo antibody [8E9] (ab252849)
    Anti-Pescadillo antibody [8E9] (ab252849)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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