Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µg 307 566 ₸ 10 µg 90 460 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Neurogranin antibody [Ng2] (ab273443)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Mouse monoclonal [Ng2] to Neurogranin
Suitable for: IHC-P, WB, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-Neurogranin antibody [Ng2]
See all Neurogranin primary antibodies
Description

Mouse monoclonal [Ng2] to Neurogranin
Host species

Mouse

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
IP	Mouse
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is considered to be commercially sensitive.
Positive control
- WB: Human brain tissue lysate; Mouse hippicampus tissue lysate; Rat hippocampus tissue lysate. IHC-P: Human, mouse and rat cerebrum tissue. IP: Mouse hippocampus tissue lysate.
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.04% PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

Ng2
Isotype

IgG2b
Research areas

Western blot - Anti-Neurogranin antibody [Ng2] (ab273443)

All lanes : Anti-Neurogranin antibody [Ng2] (ab273443) at 1/1000 dilution

Lane 1 : Human brain tissue lysate
Lane 2 : Mouse hippocampus tissue lysate
Lane 3 : Mouse spleen tissue lysate
Lane 4 : Rat hippocampus tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 8 kDa
Observed band size: 15 kDa
why is the actual band size different from the predicted?

Negative control: Mouse spleen (PMID: 20665622).

The molecular weight observed is consistent with what has been described in the literature ( PMID: 20875798).

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lane 1: 10 secs; Lanes 2-4: 3 secs.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [Ng2] (ab273443)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Neurogranin with ab273443 at 1/20000 dilution (0.053µg/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on human cerebrum tissue is observed. The section was incubated with ab273443 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 10 mins.
Immunoprecipitation - Anti-Neurogranin antibody [Ng2] (ab273443)

Neurogranin was immunoprecipitated from 0.35 mg mouse hippocampus tissue lysate with ab273443 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273443 at 1/1000 dilution. Mouse IgG for IP (HRP) (ab131368) was used at 1/10000 dilution.

Lane 1: Mouse hippocampus tissue lysate 10µg.

Lane 2: ab273443 IP in mouse hippocampus tissue lysate.

Lane 3: Mouse monoclonal IgG2b instead of ab273443 in Mouse hippocampus tissue lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 58 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [Ng2] (ab273443)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Neurogranin with ab273443 at 1/20000 dilution (0.053µg/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on mouse cerebrum tissue is observed. The section was incubated with ab273443 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 10 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [Ng2] (ab273443)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Neurogranin with ab273443 at 1/20000 dilution (0.053µg/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on rat cerebrum tissue is observed. The section was incubated with ab273443 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 10 mins.
Anti-Neurogranin antibody [Ng2] (ab273443)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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