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Neuroscience Neurology process Neurodegenerative disease Huntington's disease

Alexa Fluor® 647 Anti-GAPDH antibody [3E8AD9] (ab198105)

Alexa Fluor® 647 Anti-GAPDH antibody [3E8AD9] (ab198105)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Alexa Fluor® 647 Mouse monoclonal [3E8AD9] to GAPDH
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Isotype: IgG2b

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Overview

  • Product name

    Alexa Fluor® 647 Anti-GAPDH antibody [3E8AD9]
    See all GAPDH primary antibodies
  • Description

    Alexa Fluor® 647 Mouse monoclonal [3E8AD9] to GAPDH
  • Host species

    Mouse
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    See all applications and species data
  • Immunogen

    Tissue, cells or virus corresponding to Human GAPDH. Purified protein from Human erythrocytes.

  • Positive control

    • ICC/IF: HeLa cells.
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, PBS, 30% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purification notes

    ab110305 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity: >95% by SDS-PAGE.
  • Clonality

    Monoclonal
  • Clone number

    3E8AD9
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Huntington's disease
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Types of disease
    • Neurodegenerative disease

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GAPDH antibody [3E8AD9] (ab198105)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GAPDH antibody [3E8AD9] (ab198105)

    ab198105 staining GAPDH in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198105 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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