Anti-Carbonic Anhydrase 4/CA4 antibody (ab236315)
Key features and details
- Rabbit polyclonal to Carbonic Anhydrase 4/CA4
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Carbonic Anhydrase 4/CA4 antibody
See all Carbonic Anhydrase 4/CA4 primary antibodies -
Description
Rabbit polyclonal to Carbonic Anhydrase 4/CA4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P Human
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Immunogen
Recombinant fragment corresponding to Human Carbonic Anhydrase 4/CA4 aa 50-200.
Database link: P22748 -
Positive control
- IHC-P: Human liver cancer tissue.
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General notes
This product was previously labelled as Carbonic Anhydrase IV
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 50% Glycerol, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Carbonic Anhydrase 4/CA4 antibody (ab236315)
Immunofluorescence staining of Hela cells with CSB-PA004372LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 4/CA4 antibody (ab236315)Paraffin-embedded human liver cancer tissue stained for Carbonic Anhydrase 4/CA4 using ab236315 at 1/100 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
