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Anti-Liver Arginase antibody [C1] (ab239731)

Key features and details

  • Mouse monoclonal [C1] to Liver Arginase
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human, Pig
  • Isotype: IgG2b

Overview

  • Product name

    Anti-Liver Arginase antibody [C1]
    See all Liver Arginase primary antibodies

  • Description

    Mouse monoclonal [C1] to Liver Arginase

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    Pig
    See all applications and species data

  • Immunogen

    Recombinant full length protein (His-tag) corresponding to Human Liver Arginase aa 1-350. N-terminal His-tag (Expressed in E.coli).
    Database link: P05089

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Recombinant human Liver Arginase protein: Human, pig and mouse liver lysates: HepG2 cell lysate. IHC-P: Human liver and liver cancer tissues. ICC/IF: HepG2 cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-Liver Arginase antibody [C1] (ab239731)
    Western blot - Anti-Liver Arginase antibody [C1] (ab239731)
    Anti-Liver Arginase antibody [C1] (ab239731) at 2 µg/ml + Human liver lysate

    Secondary
    HRP-Linked goat anti-Mouse IgG at 0.2 µg/ml
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [C1] (ab239731)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [C1] (ab239731)

    Paraffin-embedded human liver tissue stained for Liver Arginase using ab239731 at 20 μg/ml in immunohistochemical analysis. DAB staining. HRP-linked Goat anti-Mouse IgG was used as the secondary antibody at 2 μg/ml.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [C1] (ab239731)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [C1] (ab239731)

    Paraffin-embedded human liver cancer tissue stained for Liver Arginase using ab239731 at 20 μg/ml in immunohistochemical analysis. DAB staining. HRP-linked Goat anti-Mouse IgG was used as the secondary antibody at 2 μg/ml.

  • Western blot - Anti-Liver Arginase antibody [C1] (ab239731)
    Western blot - Anti-Liver Arginase antibody [C1] (ab239731)
    Anti-Liver Arginase antibody [C1] (ab239731) at 2 µg/ml + Pig liver lysate

    Secondary
    HRP-Linked Goat anti-Mouse IgG at 0.2 µg/ml
  • Western blot - Anti-Liver Arginase antibody [C1] (ab239731)
    Western blot - Anti-Liver Arginase antibody [C1] (ab239731)
    Anti-Liver Arginase antibody [C1] (ab239731) at 2 µg/ml + Mouse liver lysate

    Secondary
    HRP-Linked Goat anti-Mouse IgG at 0.2 µg/ml
  • Western blot - Anti-Liver Arginase antibody [C1] (ab239731)
    Western blot - Anti-Liver Arginase antibody [C1] (ab239731)
    Anti-Liver Arginase antibody [C1] (ab239731) at 2 µg/ml + HepG2 (human liver hepatocellular carcinoma cell line) cell lysate

    Secondary
    HRP-Linked goat anti-Mouse IgG at 0.2 µg/ml
  • Immunocytochemistry/ Immunofluorescence - Anti-Liver Arginase antibody [C1] (ab239731)
    Immunocytochemistry/ Immunofluorescence - Anti-Liver Arginase antibody [C1] (ab239731)

    HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Liver Arginase using ab239731 at 40 μg/ml in ICC/IF, followed by FITC staining.

  • Western blot - Anti-Liver Arginase antibody [C1] (ab239731)
    Western blot - Anti-Liver Arginase antibody [C1] (ab239731)
    Anti-Liver Arginase antibody [C1] (ab239731) at 5 µg/ml + Recombinant human Liver Arginase protein

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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