Anti-liver Arginase antibody (ab60176)
Key features and details
- Goat polyclonal to liver Arginase
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
-
Product name
Anti-liver Arginase antibody
See all Liver Arginase primary antibodies -
Description
Goat polyclonal to liver Arginase -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Rat Liver Arginase aa 311-323 (C terminal).
Sequence:C-NHKPETDYLKPPK
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Lane 1 : Anti-liver Arginase antibody (ab60176) at 0.01 µg/ml
Lane 2 : Anti-liver Arginase antibody (ab60176) at 0.03 µg/ml
Lane 1 : Mouse liver lysate
Lane 2 : Rat liver lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 35 kDa
-
All lanes : Anti-liver Arginase antibody (ab60176) at 0.03 µg/ml
Lane 1 : Mouse Liver lysates
Lane 2 : Rat Liver lysates
Lysates/proteins at 35 µg per lane.
Predicted band size: 35 kDaSamples were incubated with prinary antibody for 1 hour. Detected by chemiluminescence.
-
Lane 1 : Anti-liver Arginase antibody (ab60176) at 0.05 µg/ml
Lane 2 : Anti-liver Arginase antibody (ab60176)
Lane 1 : Mouse Liver lysate in RIPA buffer
Lane 2 : Rat Liver lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 35 kDaDetected by chemiluminescence.
-
Anti-liver Arginase antibody (ab60176) at 1 µg/ml + Mouse liver tissue lysate in RIPA buffer at 35 µg
Predicted band size: 35 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
-
Anti-liver Arginase antibody (ab60176) at 0.03 µg/ml + Rat liver tissue lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 35 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
-
All lanes : Anti-liver Arginase antibody (ab60176) at 1/1000 dilution
Lane 1 : Tissue extract prepared from mouse liver
Lane 2 : Lysates prepared from RAW 264.7 murine macrophage cell line untreated
Lane 3 : Lysates prepared from RAW 264.7 murine macrophage cell line treated with IL-4 10 ng/mL for 48 hours
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP conjugated rabbit anti-goat polyclonal IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 seconds