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Signal Transduction Metabolism Amino Acids

Anti-liver Arginase antibody (ab60176)

Price and availability

335 040 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-liver Arginase antibody (ab60176)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Goat polyclonal to liver Arginase
  • Suitable for: WB
  • Reacts with: Mouse, Rat
  • Isotype: IgG

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Overview

  • Product name

    Anti-liver Arginase antibody
    See all Liver Arginase primary antibodies
  • Description

    Goat polyclonal to liver Arginase
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Rat Liver Arginase aa 311-323 (C terminal).
    Sequence:

    C-NHKPETDYLKPPK

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Types of disease
    • Cancer

Images

  • Western blot - Anti-liver Arginase antibody (ab60176)
    Western blot - Anti-liver Arginase antibody (ab60176)
    Lane 1 : Anti-liver Arginase antibody (ab60176) at 0.01 µg/ml
    Lane 2 : Anti-liver Arginase antibody (ab60176) at 0.03 µg/ml

    Lane 1 : Mouse liver lysate
    Lane 2 : Rat liver lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 35 kDa

  • Western blot - Anti-liver Arginase antibody (ab60176)
    Western blot - Anti-liver Arginase antibody (ab60176)
    All lanes : Anti-liver Arginase antibody (ab60176) at 0.03 µg/ml

    Lane 1 : Mouse Liver lysates
    Lane 2 : Rat Liver lysates

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 35 kDa



    Samples were incubated with prinary antibody for 1 hour. Detected by chemiluminescence.

  • Western blot - Anti-liver Arginase antibody (ab60176)
    Western blot - Anti-liver Arginase antibody (ab60176)
    Lane 1 : Anti-liver Arginase antibody (ab60176) at 0.05 µg/ml
    Lane 2 : Anti-liver Arginase antibody (ab60176)

    Lane 1 : Mouse Liver lysate in RIPA buffer
    Lane 2 : Rat Liver lysate in RIPA buffer

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 35 kDa



    Detected by chemiluminescence. 

  • Western blot - Anti-liver Arginase antibody (ab60176)
    Western blot - Anti-liver Arginase antibody (ab60176)
    Anti-liver Arginase antibody (ab60176) at 1 µg/ml + Mouse liver tissue lysate in RIPA buffer at 35 µg

    Predicted band size: 35 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-liver Arginase antibody (ab60176)
    Western blot - Anti-liver Arginase antibody (ab60176)
    Anti-liver Arginase antibody (ab60176) at 0.03 µg/ml + Rat liver tissue lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 35 kDa
    Observed band size: 37 kDa why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

  • Western blot - Anti-liver Arginase antibody (ab60176)
    Western blot - Anti-liver Arginase antibody (ab60176) This image is courtesy of an anonymous Abreview.
    All lanes : Anti-liver Arginase antibody (ab60176) at 1/1000 dilution

    Lane 1 : Tissue extract prepared from mouse liver
    Lane 2 : Lysates prepared from RAW 264.7 murine macrophage cell line untreated
    Lane 3 : Lysates prepared from RAW 264.7 murine macrophage cell line treated with IL-4 10 ng/mL for 48 hours

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP conjugated rabbit anti-goat polyclonal IgG (H+L) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 35 kDa
    Observed band size: 37 kDa why is the actual band size different from the predicted?
    Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 10 seconds

    See Abreview

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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