Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Egr1 antibody [mAbcam55160] (ab55160)

Key features and details

  • Mouse monoclonal [mAbcam55160] to Egr1
  • Suitable for: WB, IP, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-Egr1 antibody [mAbcam55160]
    See all Egr1 primary antibodies

  • Description

    Mouse monoclonal [mAbcam55160] to Egr1

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human Egr1.

    Read Abcam's proprietary immunogen policy (Peptide available as ab56196.)

  • Positive control

    • This antibody gave a positive signal in the following lysates: HeLa Whole Cell Lysate; HeLa Nuclear lysate - TPA stimulated 2 hr

  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-Egr1 antibody [mAbcam55160] (ab55160)
    Western blot - Anti-Egr1 antibody [mAbcam55160] (ab55160)
    All lanes : Anti-Egr1 antibody [mAbcam55160] (ab55160) at 5 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa nuclear extract lysate (TPA stimulated 2 hr) (ab14653)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 58 kDa
    Observed band size: 55,85 kDa
    why is the actual band size different from the predicted?
  • Immunoprecipitation - Anti-Egr1 antibody [mAbcam55160] (ab55160)
    Immunoprecipitation - Anti-Egr1 antibody [mAbcam55160] (ab55160)

    Egr1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Egr1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab55160.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 55kDa; Egr1

  • Flow Cytometry - Anti-Egr1 antibody [mAbcam55160] (ab55160)
    Flow Cytometry - Anti-Egr1 antibody [mAbcam55160] (ab55160)
    Overlay histogram showing HeLa cells stained with ab55160 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55160, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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