Human Superoxide Dismutase 3/EC-SOD ELISA Kit (ab277415)
Key features and details
- Sensitivity: 0.65 ng/ml
- Range: 0.61 ng/ml - 150 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Superoxide Dismutase 3/EC-SOD ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.65 ng/ml -
Range
0.61 ng/ml - 150 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 118.2 103% - 139% Plasma 128.3 116% - 144% Cell culture media 129.1 113% - 145% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Superoxide Dismutase 3/EC-SOD ELISA Kit (ab277415) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Superoxide Dismutase 3/EC-SOD in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Superoxide Dismutase 3/EC-SOD coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Superoxide Dismutase 3/EC-SOD present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Superoxide Dismutase 3/EC-SOD antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Superoxide Dismutase 3/EC-SOD bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated Anti-Human Superoxide Dismutase 3/EC-SOD Antibody 2 vials Anti-Human SOD3 coated Microplate 1 unit Human Superoxide Dismutase 3/EC-SOD standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen. -
Tissue specificity
Expressed in blood vessels, heart, lung, kidney and placenta. Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid. -
Sequence similarities
Belongs to the Cu-Zn superoxide dismutase family. -
Cellular localization
Secreted > extracellular space. 99% of EC-SOD is anchored to heparan sulfate proteoglycans in the tissue interstitium, and 1% is located in the vasculature in equilibrium between the plasma and the endothelium. - Information by UniProt
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Alternative names
- EC SOD
- EC-SOD
- Extracellular superoxide dismutase
see all -
Database links
- Entrez Gene: 6649 Human
- Omim: 185490 Human
- SwissProt: P08294 Human
- Unigene: 2420 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.