Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SESN1 antibody [EPR1930(2)] (ab134091)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1930(2)] to SESN1
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SESN1 antibody [EPR1930(2)]
    See all SESN1 primary antibodies

  • Description

    Rabbit monoclonal [EPR1930(2)] to SESN1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human SESN1 aa 100-200 (internal sequence). The exact sequence is proprietary.

  • Positive control

    • WB: Human heart, Ramos, K-562, JAR, HeLa, F9, C2C12, NIH/3T3, and Rat heart lysates. Flow Cyt: Daudi and permeabilized K562 cells,

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-SESN1 antibody [EPR1930(2)] (ab134091)
    Western blot - Anti-SESN1 antibody [EPR1930(2)] (ab134091)
    All lanes : Anti-SESN1 antibody [EPR1930(2)] (ab134091) at 1/1000 dilution (Purified)

    Lane 1 : Human heart lysates
    Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates
    Lane 3 : F9 (Mouse embryonal carcinoma epithelial cell) whole cell lysates
    Lane 4 : C2C12 (Mouse myoblasts myoblast) whole cell lysates
    Lane 5 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
    Lane 6 : Rat heart lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 57 kDa
    Observed band size: 65 kDa
    why is the actual band size different from the predicted?



    The bands observed are consistent with what has been described in PMID: 29751091 and PMID: 29263116 

  • Flow Cytometry - Anti-SESN1 antibody [EPR1930(2)] (ab134091)
    Flow Cytometry - Anti-SESN1 antibody [EPR1930(2)] (ab134091)

     Flow Cytometry analysis of Daudi (Human Burkitt's lymphoma lymphoblast) cells treated with 20 U/ml IFN alpha 1 for 24 hours (Red) / Untreated control (Green) labelling Anti-SESN1 with unpurified ab207414 at 1/600 dilution. Cells were fixed with 2% paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Black - isotype control, Rabbit monoclonal IgG (ab172730). Blue -unlabelled control, cells without incubation with primary and secondary antibodies.

  • Western blot - Anti-SESN1 antibody [EPR1930(2)] (ab134091)
    Western blot - Anti-SESN1 antibody [EPR1930(2)] (ab134091)
    Lanes 1 & 3-7 : Anti-SESN1 antibody [EPR1930(2)] (ab134091) at 1/1000 dilution ((unpurified))
    Lane 2 : Anti-SESN1 antibody [EPR1930(2)] (ab134091) at 1/1000 dilution

    Lane 1 : Ramos cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : C2C12 cell lysate
    Lane 5 : NIH3T3 cell lysate
    Lane 6 : F9 cell lysate
    Lane 7 : JAR cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 57 kDa

  • Flow Cytometry - Anti-SESN1 antibody [EPR1930(2)] (ab134091)
    Flow Cytometry - Anti-SESN1 antibody [EPR1930(2)] (ab134091)

    Flow Cytometry analysis of permeabilized K562 cells labeling SESN1 with unpurified ab134091 at 1/100 dilution (red). Rabbit IgG (negative control) shown in green.

  • OI-RD Scanning - Anti-SESN1 antibody [EPR1930(2)] (ab134091)
    OI-RD Scanning - Anti-SESN1 antibody [EPR1930(2)] (ab134091)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-SESN1 antibody [EPR1930(2)] (ab134091)
    Anti-SESN1 antibody [EPR1930(2)] (ab134091)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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