Mouse skin tissue lysate - total protein (ab4025)
Overview
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Product name
Mouse skin tissue lysate - total protein
See all Skin lysates -
General notes
Source: Outbred NIH-Swiss Albino mice.Mouse skin tissue lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecyl sulfate (SDS), 1 mM sodium ethylene diamine tetra acetate, 1 mM phenyl methyl sulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% Beta-mercaptoethanol.
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituents: 60.05% Water, 12.5% Glycerol (glycerin, glycerine), 9% Tris HCl, 7.7% DTT, 4.4% Sodium chloride, 1% Triton-X-100, 1% Sodium deoxycholate, 1.1% Sodium lauryl sulfate, 0.15% EDTA disodium salt, 0.5% Aprotinin, 0.5% Leupeptin hemisulfate, 0.09% PMSF, 0.01% Bromophenol blue -
Concentration information loading... -
Lysate notes
Mouse skin tissue lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecyl sulfate (SDS), 1 mM sodium ethylene diamine tetra acetate, 1 mM phenyl methyl sulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% Beta-mercaptoethanol. -
Research areas