Anti-Calreticulin antibody (ab265598)
Key features and details
- Rabbit polyclonal to Calreticulin
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Calreticulin antibody
See all Calreticulin primary antibodies -
Description
Rabbit polyclonal to Calreticulin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human Calreticulin aa 367-417. The exact sequence is proprietary. NP_004334.1
Database link: P27797 -
Positive control
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysate. IP: HeLa whole cell lysate.
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General notes
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Calreticulin antibody (ab265598)All lanes : Anti-Calreticulin antibody (ab265598) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T whole cell lysate at 50 µg
Lane 5 : NIH/3T3 whole cell lysate at 50 µg
Predicted band size: 48 kDa
Exposure time: 1 second
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Immunoprecipitation - Anti-Calreticulin antibody (ab265598)Calreticulin was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab265598 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab265598 at 0.1 µg/ml.
Lane 1: ab265598 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Exposure time: 1 sec.
