Anti-FANCC antibody (ab5065)
Key features and details
- Rabbit polyclonal to FANCC
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FANCC antibody
See all FANCC primary antibodies -
Description
Rabbit polyclonal to FANCC -
Host species
Rabbit -
Specificity
Detects a band at 60kDa in Hela cell lysate corresponding to FANCC. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide:
CLINKEPQNSGSKLNS
, corresponding to amino acids 96 - 112 of Human FANCC.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.878% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FANCC antibody (ab5065)Anti-FANCC antibody (ab5065) at 1/1500 dilution + HeLa whole cell lysate at 35 µg
Secondary
IRDye 800 conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilution
Predicted band size: 63 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Additional bands at: 33 kDa. We are unsure as to the identity of these extra bands.
Primary antibody incubated with PBS supplemented with 1% normal goat serum and 0.1% milk. -
Immunocytochemistry/ Immunofluorescence - Anti-FANCC antibody (ab5065)ICC/IF image of ab5065 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5065, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
