Anti-beta 2 Microglobulin antibody [B2M-01] (ab759)
![Anti-beta 2 Microglobulin antibody [B2M-01] (ab759)](https://www.abcam.com/ps/products/0/ab759/Images/ab759-3701-anti-beta-2-microglobulin-antibody-b2m-01-western-blot.jpg "Anti-beta 2 Microglobulin antibody [B2M-01] (ab759)")
Key features and details
- Mouse monoclonal [B2M-01] to beta 2 Microglobulin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-beta 2 Microglobulin antibody [B2M-01]
See all beta 2 Microglobulin primary antibodies -
Description
Mouse monoclonal [B2M-01] to beta 2 Microglobulin -
Host species
Mouse -
Specificity
This antibody reacts with beta-2-microglobulin, associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble forms of beta-2-microglobulin. The antibody detected beta-2-microglobulin in U-937 and RAJI cell lines in WB in non reducing conditions however failed to detect a band in JEG-3 and A431 cell lysates. All lysates gave a signal in direct ELISA. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rabbit, Chicken, Cow, Dog, Pig -
Immunogen
Full length native protein (purified) (Human).
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Positive control
- U-937 and RAJI cell lysates
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
Purified by precipitation methods. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
B2M-01 -
Isotype
IgG2a -
Research areas
Images
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Western blot - Anti-beta 2 Microglobulin antibody [B2M-01] (ab759)All lanes : Anti-beta 2 Microglobulin antibody [B2M-01] (ab759) at 2 µg/ml
Lane 1 : JEG-3 cell lysate
Lane 2 : A-431 cell lysate
Lane 3 : U-937 cell lysate
Lane 4 : Raji cell lysate
Developed using the ECL technique.
Performed under non-reducing conditions.
Predicted band size: 13 kDa
Lysates were separated on 15% SDS–PAGE gel and electrotransferred to a PVDF membrane. The membrane was blocked in PBS/Tween buffer containing 5% non fat dry milk for 2 hours and probed with ab759 at 4°C overnight.
