Human Lactoferrin ELISA Kit (ab200015)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 48.8 pg/ml
- Range: 156.3 pg/ml - 10000 pg/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Milk, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Lactoferrin Capture Antibody 1 x 600µl 10X Human Lactoferrin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human Lactoferrin Lyophilized Purified Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.
Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.
Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.
The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity. -
Sequence similarities
Belongs to the transferrin family.
Contains 2 transferrin-like domains. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- GIG12
- Growth inhibiting protein 12
- HLF2
see all -
Database links
- Entrez Gene: 4057 Human
- Omim: 150210 Human
- SwissProt: P02788 Human
- Unigene: 529517 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Lactoferrin standard curve in Sample Diluent NS.The Lactoferrin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Standard curveExample of human Lactoferrin standard curve in Sample Diluent NS. The Lactoferrin standard curve was prepared. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Lactoferrin in human serum and plasma samples.The concentrations of Lactoferrin were measured in duplicates, interpolated from the Lactoferrin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:200, plasma (citrate) 1:200, plasma (heparin) 1:200, and plasma (EDTA) 1:200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lactoferrin concentration was determined to be 1,142 ng/mL in neat serum, 885.1 ng/mL in neat plasma (citrate), 1,054 ng/mL in neat plasma (heparin), and 581.4 ng/mL in neat plasma (EDTA).
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Interpolated concentrations of native Lactoferrin in human milk (defatted), urine, and saliva samples.The concentrations of Lactoferrin were measured in duplicates, interpolated from the Lactoferrin standard curves and corrected for sample dilution. Undiluted samples are as follows: milk 1:500,000, urine 1:20, and saliva 1:10,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lactoferrin concentration was determined to be 4,021.6 µg/mL in neat milk (defatted), 0.033 µg/mL in neat urine, and 39.1 µg/mL in neat saliva.
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lactoferrin concentration was determined to be 473.8 ng/mL with a range of 315.2 – 852.7 ng/mL.
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Linearity of dilution.Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native Lactoferrin was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
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Linearity of dilution.Native Lactoferrin was measured in human milk, urine, and saliva samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
Native purified Lactoferrin protein was spiked into RPMI 1640 cell culture media containing 10% fetal bovine serum and measured in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
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Sandwich ELISA - Human Lactoferrin ELISA Kit (ab200015)To learn more about the advantages of recombinant antibodies see here.
