Human Lactoferrin ELISA Kit (HLF2) (ab108882)
Key features and details
- Sensitivity: 0.35 ng/ml
- Range: 0.625 ng/ml - 40 ng/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Lactoferrin ELISA Kit (HLF2)
See all Lactoferrin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 7.2% Inter-assay Sample n Mean SD CV% Overall 9.2% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid -
Assay type
Sandwich (quantitative) -
Sensitivity
= 0.35 ng/ml -
Range
0.625 ng/ml - 40 ng/ml -
Recovery
= 102 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Lactoferrin (HLF2) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Lactoferrin levels in Human cell culture supernatants, serum, urine, saliva, milk, cerebrospinal fluid and plasma.
A Lactoferrin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Lactoferrin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Lactoferrin captured in plate.
Get results in 90 minutes with Human Lactoferrin ELISA Kit (ab200015) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Lactoferrin Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Lactoferrin Microplate (12 x 8 well strips) 1 unit Lactoferrin Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
Research areas
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Function
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.
Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.
Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.
The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity. -
Sequence similarities
Belongs to the transferrin family.
Contains 2 transferrin-like domains. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- GIG12
- Growth inhibiting protein 12
- HLF2
see all -
Database links
- Entrez Gene: 4057 Human
- Omim: 150210 Human
- SwissProt: P02788 Human
- Unigene: 529517 Human
Images
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Sandwich ELISA - Lactoferrin (HLF2) Human ELISA Kit (ab108882)Lactoferrin measured in biological fluids with background signal subtracted (duplicates +/- SD).
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Typical Standard CurveRepresentative Standard Curve Using ab108882.
