Human Albumin ELISA Kit (ab227933)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 30 pg/ml
- Range: 312.5 pg/ml - 20000 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Albumin ELISA Kit
See all Albumin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 9 4% Inter-assay Sample n Mean SD CV% Serum 3 12.6% -
Sample type
Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
30 pg/ml -
Range
312.5 pg/ml - 20000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 104 101% - 107% Serum 117 106% - 133% Hep Plasma 111 104% - 116% EDTA Plasma 115 108% - 121% Cit plasma 112 106% - 117% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow, Rhesus monkey -
Product overview
Human Albumin ELISA Kit (ab227933) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Albumin protein in cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Albumin with 30 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
CROSS REACTIVITY Purified mouse, bovine, and cynomolgus monkey serum albumin were prepared at 1,000,1000, and 500 ng/mL, respectively, and assayed for cross reactivity. No cross-reactivity was observed.
SPECIES REACTIVITY This kit recognizes Human Albumin protein. Other species reactivity was determined by measuring 1:5 million serum or plasma samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum or plasma assayed at the same dilution. Reactivity -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Albumin Capture Antibody 1 x 600µl 10X Human Albumin Detector Antibody 1 x 600µl Native Human Serum Albumin protein (Lyophilized) (ab205808) 2 vials Antibody Diluent CP 1 x 6ml 10X Wash Buffer PT (ab206977) 1 x 20ml TMB Development Solution 1 x 12ml Stop Solution 1 x 12ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Plate Seals 1 unit -
Research areas
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Function
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. -
Tissue specificity
Plasma. -
Involvement in disease
Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population. -
Sequence similarities
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
Post-translational
modificationsKenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- alb
- ALBU_HUMAN
- Albumin (32 AA)
see all -
Database links
- Entrez Gene: 213 Human
- Omim: 103600 Human
- SwissProt: P02768 Human
- Unigene: 418167 Human
- Unigene: 592379 Human
Images
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Other - Human Albumin ELISA Kit (ab227933)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Albumin standard curve in Sample Diluent NSBackground-subtracted data values (mean +/- SD) are graphed.
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Standard curveExample of human Albumin standard curve in Sample Diluent NS. The Albumin standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Albumin in human serum and plasma samplesThe concentrations of Serum Albumin were measured in duplicates, interpolated from the Serum Albumin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:5 million, plasma (citrate) 1:5 million, plasma (heparin) 1:5 million, and plasma (EDTA) 1:10 million. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Serum Albumin concentration was determined to be 39.3 mg/mL in serum, 34.4 mg/mL in plasma (citrate), 30.8 mg/mL in plasma (heparin), and 34.5 mg/mL in plasma (EDTA).
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Interpolated concentrations of native Albumin in human urine samplesThe concentrations of Serum Albumin were measured in duplicates, interpolated from the Serum Albumin standard curves and corrected for sample dilution. Undiluted samples are 1:500. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Serum Albumin concentration was determined to be 6,290 ng/mL in urine.
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Serum from ten individual healthy human female donors was measured in duplicateInterpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Serum Albumin concentration was determined to be 33.31 mg/mL with a range of 22.5 – 46.2 mg/mL.
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Linearity of dilution.Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native Albumin was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
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Sandwich ELISA - Human Albumin ELISA Kit (ab227933)To learn more about the advantages of recombinant antibodies see here.
