Human Albumin ELISA Kit (ab179887)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.1 ng/ml
- Range: 0.7 ng/ml - 45 ng/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Albumin ELISA Kit
See all Albumin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 5.2% Inter-assay Sample n Mean SD CV% Serum 3 7.6% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.1 ng/ml -
Range
0.7 ng/ml - 45 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 104 104% - 105% Serum 100 92% - 110% Hep Plasma 98 89% - 105% EDTA Plasma 104 97% - 109% Cit plasma 101 100% - 103% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
As of December 12, 2019 Human Albumin ELISA kit has been re-developed with new recombinant monoclonal antibodies to provide improved performance and consistency.
Human Albumin ELISA kit (ab179887) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Albumin protein in human serum and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate human Albumin with 100 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.ASSAY SPECIFICITY
This kit recognizes native human Albumin protein in serum, plasma, and cell culture supernatant samples only. Urine, saliva, cell and tissue extract samples have not been tested with this kit.
SPECIES REACTIVITY
This kit recognizes human Albumin and Cynomolgus monkey albumin protein.
Other species reactivity was determined by measuring 1:4,000,000 serum samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum assayed at the same dilution.
Reactivity
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Notes
Albumin (ALB), the main protein of plasma, has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. It also serves as a major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Albumin Capture Antibody 1 x 600µl 10X Human Albumin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Native Human Serum Albumin protein (Lyophilized) (ab205808) 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 2 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. -
Tissue specificity
Plasma. -
Involvement in disease
Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population. -
Sequence similarities
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
Post-translational
modificationsKenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- alb
- ALBU_HUMAN
- Albumin (32 AA)
see all -
Database links
- Entrez Gene: 213 Human
- Omim: 103600 Human
- SwissProt: P02768 Human
- Unigene: 418167 Human
- Unigene: 592379 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human Albumin standard curve comparisonStandard Curve comparison between the original human Albumin SimpleStep ELISA kit current human Albumin SimpleStep ELISA kit.
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Standard curveBackground-subtracted data values (mean +/- SD) are graphed.
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Standard curveExample of human Albumin standard curve in Sample Diluent NS. The Albumin standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Albumin in human serum, plasma and cell culture supernatant samples.The concentrations of Albumin were measured in duplicates, interpolated from the Albumin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:4,000,000, plasma (EDTA) 1:4,000,000, plasma (citrate) 1:8,000,000, and plasma (heparin) 1:8,000,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Albumin concentration was determined to be 31.5 mg/mL in serum, 75.4 mg/mL in plasma (EDTA), 35.5 mg/mL in plasma (citrate) and 41.3 mg/mL in plasma (heparin).
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Serum from eight individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Albumin concentration was determined to be 23.4 mg/mL with a range of 10.5 – 37.1 mg/mL.
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Linearity of dilution for spiked albuminLinearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Spiked Albumin was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
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Linearity of dilution for albuminLinearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native Albumin was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
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Sandwich ELISA - Human Albumin ELISA Kit (ab179887)To learn more about the advantages of recombinant antibodies see here.
