Human Albumin Antibody Pair - BSA and Azide free (ab256595)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human, Cynomolgus monkey, Rhesus monkey
Overview
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Product name
Human Albumin Antibody Pair - BSA and Azide free
See all Albumin kits -
Assay type
ELISA set -
Range
0.31 ng/ml - 20 ng/ml -
Species reactivity
Reacts with: Human, Cynomolgus monkey, Rhesus monkey -
Product overview
The Antibody Pair can be used to quantify Human Albumin. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab179887), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Human Albumin Capture Antibody (unconjugated) 1 x 100µg Human Albumin Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. -
Tissue specificity
Plasma. -
Involvement in disease
Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population. -
Sequence similarities
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
Post-translational
modificationsKenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- alb
- ALBU_HUMAN
- Albumin (32 AA)
see all -
Database links
- Entrez Gene: 213 Human
- Omim: 103600 Human
- SwissProt: A2V9Z4 Cynomolgus monkey
- SwissProt: P02768 Human
- Unigene: 418167 Human
- Unigene: 592379 Human
Images
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Sandwich ELISA - Human Albumin Antibody Pair - BSA and Azide free (ab256595)To learn more about the advantages of recombinant antibodies see here.
