Bovine Lactoferrin ELISA Kit (ab274406)
Key features and details
- Range: 3.13 ng - 200 ng
- Sample type: Milk, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Cow
Overview
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Product name
Bovine Lactoferrin ELISA Kit
See all Lactoferrin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Milk, Urine, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Range
3.13 ng - 200 ng -
Recovery
Sample specific recovery Sample type Average % Range Serum > 85 % - % -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Cow -
Product overview
The Bovine Lactoferrin ELISA kit (ab274406) is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Lactoferrin (LF) in bovine biological samples.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Buffer Concentrate 1 x 50ml Antibody coated plate 1 unit Bovine Lactoferrin Calibrator (Lyophilized) 1 vial 100X HRP-conjugated (Bovine Lactoferrin) antibody 1 vial Stop Solution 1 x 12ml Chromogen Substrate Solution 1 x 12ml -
Research areas
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Function
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.
Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.
Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.
The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity. -
Sequence similarities
Belongs to the transferrin family.
Contains 2 transferrin-like domains. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- GIG12
- Growth inhibiting protein 12
- HLF2
see all -
Database links
- Entrez Gene: 280846 Cow
- SwissProt: P24627 Cow
Images
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Example standard curve
Example standard curve using the Bovine Lactoferrin ELISA Kit (ab274406).