Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD83 antibody [EPR22405] (ab244204)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22405] to CD83
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CD83 antibody [EPR22405]
    See all CD83 primary antibodies

  • Description

    Rabbit monoclonal [EPR22405] to CD83

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: LPS treated THP-1 whole cell lysate. Raji and HDLM-2 whole cell lysate (Treated/untreated with PNGase F). ICC/IF: HDLM-2 cells. Flow Cyt: Human PBMC and HDLM-2 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CD83 antibody [EPR22405] (ab244204)
    Western blot - Anti-CD83 antibody [EPR22405] (ab244204)
    All lanes : Anti-CD83 antibody [EPR22405] (ab244204) at 1/1000 dilution

    Lane 1 : Untreated Raji (human burkitt's lymphoma b lymphocyte) whole cell lysate
    Lane 2 : Raji whole cell lysate treated with PNGase F
    Lane 3 : Untreated HDLM-2 (human hodgkin lymphoma) whole cell lysate
    Lane 4 : HDLM-2 whole cell lysate treated with PNGase F

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 23 kDa
    Observed band size: 40-50 kDa
    why is the actual band size different from the predicted?



    The molecular weight observed is consistent with what has been described in the literature (PMID: 15320871). The bands above 23 kDa are glycosylated forms.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 26 seconds.

  • Western blot - Anti-CD83 antibody [EPR22405] (ab244204)
    Western blot - Anti-CD83 antibody [EPR22405] (ab244204)
    All lanes : Anti-CD83 antibody [EPR22405] (ab244204) at 1/1000 dilution

    Lane 1 : Untreated THP-1 (human monocytic leukemia monocyte) whole cell lysate
    Lane 2 : THP-1 treated with 100ng/ml LPS for 3 hours, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 23 kDa



    The molecular weight observed is consistent with what has been described in the literature (PMID: 15320871). The bands above 23 kDa are glycosylated forms.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Flow Cytometry - Anti-CD83 antibody [EPR22405] (ab244204)
    Flow Cytometry - Anti-CD83 antibody [EPR22405] (ab244204)

    Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) labeling CD83 with ab244204 at 1/50 (Right panel) compared with a Rabbit monoclonal IgG (ab172730) (Left panel). Goat anti rabbit IgG (Dylight® 488, ab98462), at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG (Left panel) or anti-CD83 RabMab (Right panel). Then stained with Alexa Fluor® 647-conjugated anti-CD14. The result is consistent with what has been described in the literature. (PMID: 15320871).

    Gated on viable cells.

  • Flow Cytometry - Anti-CD83 antibody [EPR22405] (ab244204)
    Flow Cytometry - Anti-CD83 antibody [EPR22405] (ab244204)

    Flow cytometric analysis of HDLM-2 (human Hodgkin lymphoma) cell line labeling CD83 with ab244204 at 1/50 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

    Gated on viable cells.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD83 antibody [EPR22405] (ab244204)
    Immunocytochemistry/ Immunofluorescence - Anti-CD83 antibody [EPR22405] (ab244204)

    Immunofluorescent analysis of HDLM-2 (human Hodgkin lymphoma) cells fixed with 100% methanol, labeling CD83 with ab244204 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous and cytoplasmic staining in HDLM-2 cells is observed. We recommend using methanol instead of PFA fixation. The nuclear counter stain is DAPI (blue).
    Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red).
    The negative control is the secondary antibody only.

  • Anti-CD83 antibody [EPR22405] (ab244204)
    Anti-CD83 antibody [EPR22405] (ab244204)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-CD83 antibody [EPR22405] (ab244204)

© 2021 Astana Biomed Group LLP. All rights reserved.