Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Tuesday March 09, 2021

Anti-CD83 antibody [EPR22405] (ab244204)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22405] to CD83
Suitable for: WB, ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-CD83 antibody [EPR22405]
See all CD83 primary antibodies
Description

Rabbit monoclonal [EPR22405] to CD83
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: LPS treated THP-1 whole cell lysate. Raji and HDLM-2 whole cell lysate (Treated/untreated with PNGase F). ICC/IF: HDLM-2 cells. Flow Cyt: Human PBMC and HDLM-2 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22405
Isotype

IgG
Research areas

Western blot - Anti-CD83 antibody [EPR22405] (ab244204)

All lanes : Anti-CD83 antibody [EPR22405] (ab244204) at 1/1000 dilution

Lane 1 : Untreated Raji (human burkitt's lymphoma b lymphocyte) whole cell lysate
Lane 2 : Raji whole cell lysate treated with PNGase F
Lane 3 : Untreated HDLM-2 (human hodgkin lymphoma) whole cell lysate
Lane 4 : HDLM-2 whole cell lysate treated with PNGase F

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 23 kDa
Observed band size: 40-50 kDa
why is the actual band size different from the predicted?

The molecular weight observed is consistent with what has been described in the literature (PMID: 15320871). The bands above 23 kDa are glycosylated forms.

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: 26 seconds.
Western blot - Anti-CD83 antibody [EPR22405] (ab244204)

All lanes : Anti-CD83 antibody [EPR22405] (ab244204) at 1/1000 dilution

Lane 1 : Untreated THP-1 (human monocytic leukemia monocyte) whole cell lysate
Lane 2 : THP-1 treated with 100ng/ml LPS for 3 hours, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 23 kDa

The molecular weight observed is consistent with what has been described in the literature (PMID: 15320871). The bands above 23 kDa are glycosylated forms.

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.
Flow Cytometry - Anti-CD83 antibody [EPR22405] (ab244204)

Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) labeling CD83 with ab244204 at 1/50 (Right panel) compared with a Rabbit monoclonal IgG (ab172730) (Left panel). Goat anti rabbit IgG (Dylight^® 488, ab98462), at 1/2000 dilution was used as the secondary antibody.

Cells were stained with rabbit IgG (Left panel) or anti-CD83 RabMab (Right panel). Then stained with Alexa Fluor^® 647-conjugated anti-CD14. The result is consistent with what has been described in the literature. (PMID: 15320871).

Gated on viable cells.
Flow Cytometry - Anti-CD83 antibody [EPR22405] (ab244204)

Flow cytometric analysis of HDLM-2 (human Hodgkin lymphoma) cell line labeling CD83 with ab244204 at 1/50 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

Gated on viable cells.
Immunocytochemistry/ Immunofluorescence - Anti-CD83 antibody [EPR22405] (ab244204)

Immunofluorescent analysis of HDLM-2 (human Hodgkin lymphoma) cells fixed with 100% methanol, labeling CD83 with ab244204 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous and cytoplasmic staining in HDLM-2 cells is observed. We recommend using methanol instead of PFA fixation. The nuclear counter stain is DAPI (blue).
Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at a 1/200 dilution (red).
The negative control is the secondary antibody only.
Anti-CD83 antibody [EPR22405] (ab244204)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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