Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD83 antibody [EPR22405] - BSA and Azide free (ab245986)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22405] to CD83 - BSA and Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CD83 antibody [EPR22405] - BSA and Azide free
    See all CD83 primary antibodies

  • Description

    Rabbit monoclonal [EPR22405] to CD83 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HDLM-2 cells. Flow Cyt: Human PBMC and HDLM-2 cells.

  • General notes

    Ab245986 is the carrier-free version of ab244204. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab245986 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Flow Cytometry - Anti-CD83 antibody [EPR22405] - BSA and Azide free (ab245986)
    Flow Cytometry - Anti-CD83 antibody [EPR22405] - BSA and Azide free (ab245986)

    Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) labeling CD83 with ab244204 at 1/50 (Right panel) compared with a Rabbit monoclonal IgG (ab172730) (Left panel). Goat anti rabbit IgG (Dylight® 488, ab98462), at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG (Left panel) or anti-CD83 RabMab (Right panel). Then stained with Alexa Fluor® 647-conjugated anti-CD14. The result is consistent with what has been described in the literature. (PMID: 15320871).

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab244204).

  • Flow Cytometry - Anti-CD83 antibody [EPR22405] - BSA and Azide free (ab245986)
    Flow Cytometry - Anti-CD83 antibody [EPR22405] - BSA and Azide free (ab245986)

    Flow cytometric analysis of HDLM-2 (human Hodgkin lymphoma) cell line labeling CD83 with ab244204 at 1/50 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab244204).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD83 antibody [EPR22405] - BSA and Azide free (ab245986)
    Immunocytochemistry/ Immunofluorescence - Anti-CD83 antibody [EPR22405] - BSA and Azide free (ab245986)

    Immunofluorescent analysis of HDLM-2 (human Hodgkin lymphoma) cells fixed with 100% methanol, labeling CD83 with ab244204 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous and cytoplasmic staining in HDLM-2 cells. We recommend using methanol instead of PFA fixation. The nuclear counter stain is DAPI (blue).
    Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red).
    The negative control is the secondary antibody only.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab244204).
  • Anti-CD83 antibody [EPR22405] - BSA and Azide free (ab245986)
    Anti-CD83 antibody [EPR22405] - BSA and Azide free (ab245986)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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