Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD7 antibody [EPR4242] - BSA and Azide free (ab230834)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4242] to CD7 - BSA and Azide free
  • Suitable for: ICC, WB, IP, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CD7 antibody [EPR4242] - BSA and Azide free
    See all CD7 primary antibodies

  • Description

    Rabbit monoclonal [EPR4242] to CD7 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the IHC-P results. We do not guarantee WB for mouse and rat.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: Jurkat cells; IP: Human spleen lysate; ICC: Jurkat cells; IHC-P: Rat and mouse spleen tissues. Human colon carcinoma and tonsil tissues; WB: Human spleen lysate,

  • General notes

    ab230834 is the carrier-free version of ab109296 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab230834 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Dissociation constant (KD)

    KD = 2.40 x 10 -12 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR4242

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-CD7 antibody [EPR4242] - BSA and Azide free (ab230834)
    Western blot - Anti-CD7 antibody [EPR4242] - BSA and Azide free (ab230834)
    Anti-CD7 antibody [EPR4242] (ab109296) at 1/10000 dilution + Human spleen lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 25 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab109296, the same antibody clone in a different buffer formulation.

    The molecular mass observed is consistent with what has been described in the literatures (PMID: 24157461, 2479685).

  • Flow Cytometry - Anti-CD7 antibody [EPR4242] - BSA and Azide free (ab230834)
    Flow Cytometry - Anti-CD7 antibody [EPR4242] - BSA and Azide free (ab230834)

    This data was developed using ab109296, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Jurkat (Human T cell leukemia T lymphocyte) cells labelling CD7 with purified ab109296 at 1/100 dilution (1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [EPR4242] - BSA and Azide free (ab230834)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [EPR4242] - BSA and Azide free (ab230834)
    This data was developed using ab109296, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat spleen tissue sections labeling CD7 with Purified ab109296 at 1/8000 dilution (0.07 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunocytochemistry - Anti-CD7 antibody [EPR4242] - BSA and Azide free (ab230834)
    Immunocytochemistry - Anti-CD7 antibody [EPR4242] - BSA and Azide free (ab230834)
    This data was developed using ab109296, the same antibody clone in a different buffer formulation.
    Immunocytochemistry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling CD7 with Purified ab109296 at 1/50 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 dilution (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/mL). DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [EPR4242] - BSA and Azide free (ab230834)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [EPR4242] - BSA and Azide free (ab230834)
    This data was developed using ab109296, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse spleen tissue sections labeling CD7 with Purified ab109296 at 1/8000 dilution (0.07 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [EPR4242] - BSA and Azide free (ab230834)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [EPR4242] - BSA and Azide free (ab230834)
    This data was developed using ab109296, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue sections labeling CD7 with Purified ab109296 at 1/8000 dilution (0.07 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [EPR4242] - BSA and Azide free (ab230834)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [EPR4242] - BSA and Azide free (ab230834)
    This data was developed using ab109296, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling CD7 with Purified ab109296 at 1/8000 dilution (0.07 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunoprecipitation - Anti-CD7 antibody [EPR4242] - BSA and Azide free (ab230834)
    Immunoprecipitation - Anti-CD7 antibody [EPR4242] - BSA and Azide free (ab230834)

    This data was developed using ab109296, the same antibody clone in a different buffer formulation.

    CD7 was immunoprecipitated from 0.35 mg Human spleen lysate 10 ug with purified ab109296 at 1/50. Western blot was performed on the immunoprecipitate using ab109296 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Human spleen lysate 10 ug

    Lane 2: ab109296 IP in Human spleen lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab109296 in Human spleen lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds

  • OI-RD Scanning - Anti-CD7 antibody [EPR4242] - BSA and Azide free (ab230834)
    OI-RD Scanning - Anti-CD7 antibody [EPR4242] - BSA and Azide free (ab230834)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109296).

  • Anti-CD7 antibody [EPR4242] - BSA and Azide free (ab230834)
    Anti-CD7 antibody [EPR4242] - BSA and Azide free (ab230834)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-CD7 antibody [EPR4242] - BSA and Azide free (ab230834)

© 2021 Astana Biomed Group LLP. All rights reserved.