Anti-Spermine synthase antibody [EPR9253] - BSA and Azide free (ab248996)
![Anti-Spermine synthase antibody [EPR9253] - BSA and Azide free (ab248996)](https://www.abcam.com/ps/products/248/ab248996/Images/ab248996-406428-anti-spermine-synthase-antibody-epr9253-bsa-and-azide-free-western-blot.jpg "Anti-Spermine synthase antibody [EPR9253] - BSA and Azide free (ab248996)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9253] to Spermine synthase - BSA and Azide free
- Suitable for: WB, Flow Cyt
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Spermine synthase antibody [EPR9253] - BSA and Azide free
See all Spermine synthase primary antibodies -
Description
Rabbit monoclonal [EPR9253] to Spermine synthase - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab248996 is the carrier-free version of ab151547. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab248996 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR9253 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Spermine synthase antibody [EPR9253] - BSA and Azide free (ab248996)
This data was developed using ab151547, the same antibody clone in a different buffer formulation.
Lane 1 Wild-type HAP1 cell lysate (20 µg)
Lane 2 Spermine synthase knockout HAP1 cell lysate (20 µg)
Lane 3 HeLa cell lysate (20 µg)
Lanes 1 - 3 Merged signal (red and green). Green - ab151547 observed at 40 kDa. Red - loading control, ab18058, observed at 124 kDa.
ab151547 was shown to specifically react with Spermine synthase when Spermine synthase knockout samples were used. Wild-type and Spermine synthase knockout samples were subjected to SDS-PAGE. ab151547 and ab18058 (loading control to vinculin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Spermine synthase antibody [EPR9253] - BSA and Azide free (ab248996)](https://www.abcam.com/ps/products/248/ab248996/Images/ab248996-402267-anti-spermine-synthase-antibody-epr9253-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Spermine synthase antibody [EPR9253] - BSA and Azide free (ab248996)All lanes : Anti-Spermine synthase antibody [EPR9253] (ab151547) at 1/1000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : HeLa lysate
Lane 3 : Jurkat lysate
Lane 4 : K562 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 41 kDaThis data was developed using ab151547, the same antibody clone in a different buffer formulation.
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![Flow Cytometry - Anti-Spermine synthase antibody [EPR9253] - BSA and Azide free (ab248996)](https://www.abcam.com/ps/products/248/ab248996/Images/ab248996-1-anti-spermine-synthase-antibody-epr9253-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-Spermine synthase antibody [EPR9253] - BSA and Azide free (ab248996) -
![Anti-Spermine synthase antibody [EPR9253] - BSA and Azide free (ab248996)](https://www.abcam.com/ps/products/248/ab248996/Images/ab248996-4-benefits-of-recombinant-antibodies.png)
Anti-Spermine synthase antibody [EPR9253] - BSA and Azide free (ab248996)