Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Drosha antibody [EPR12794] - BSA and Azide free (ab227460)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12794] to Drosha - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Drosha antibody [EPR12794] - BSA and Azide free
    See all Drosha primary antibodies

  • Description

    Rabbit monoclonal [EPR12794] to Drosha - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat, HEK-293T, and HeLa cell lysates. IHC-P: Human lung adenocarcinoma tissue. ICC/IF: HeLa cells. Flow Cyt: Jurkat cells.

  • General notes

    ab227460 is the carrier-free version of ab183732. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab227460 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-Drosha antibody [EPR12794] - BSA and Azide free (ab227460)
    Western blot - Anti-Drosha antibody [EPR12794] - BSA and Azide free (ab227460)
    All lanes : Anti-Drosha antibody [EPR12794] (ab183732) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : DROSHA knockout HEK-293T cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 159 kDa
    Observed band size: 159 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab183732).

    Lanes 1-3: Merged signal (red and green). Green - ab183732 observed at 159 kDa. Red - loading control, ab8245 observed at 37 kDa.

    ab183732 Anti-Drosha antibody [EPR12794] was shown to specifically react with Drosha in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266217 (knockout cell lysate ab257171) was used. Wild-type and Drosha knockout samples were subjected to SDS-PAGE. ab183732 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

     

     

  • Flow Cytometry - Anti-Drosha antibody [EPR12794] - BSA and Azide free (ab227460)
    Flow Cytometry - Anti-Drosha antibody [EPR12794] - BSA and Azide free (ab227460)

    Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling Drosha with purified ab183732 at 1/230 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183732).

  • Immunocytochemistry/ Immunofluorescence - Anti-Drosha antibody [EPR12794] - BSA and Azide free (ab227460)
    Immunocytochemistry/ Immunofluorescence - Anti-Drosha antibody [EPR12794] - BSA and Azide free (ab227460)

    Immunocytochemistry/Immunofluorescence analysis of Jurkat cells labelling Drosha with ab183732 at 1/500. Cells were fixed with 4% Paraformaldehyde. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.

    Control: PBS only.
    Nuclear counter stain: DAPI.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183732).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Drosha antibody [EPR12794] - BSA and Azide free (ab227460)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Drosha antibody [EPR12794] - BSA and Azide free (ab227460)

    This IHC data was generated using the same anti-Drosha antibody clone, EPR12794, in a different buffer formulation (cat# ab183732).

    Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling Drosha with ab183732 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-Drosha antibody [EPR12794] - BSA and Azide free (ab227460)
    Anti-Drosha antibody [EPR12794] - BSA and Azide free (ab227460)

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