Anti-Drosha antibody (ab58589)
Key features and details
- Goat polyclonal to Drosha
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Drosha antibody
See all Drosha primary antibodies -
Description
Goat polyclonal to Drosha -
Host species
Goat -
Specificity
This antibody also cross-reacts with human Ribonuclease III (GeneID 29102; NP_037367.2). -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide:
C-KQTDKQKLAQRE
, corresponding to internal sequence amino acids 811-822 of Human Drosha -
Positive control
- Human Liver lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Drosha antibody (ab58589)Anti-Drosha antibody (ab58589) at 0.5 µg/ml (incubated for 1 hour) + Human Liver lysate (35µg protein in RIPA buffer).
Developed using the ECL technique.
Predicted band size: 159 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Drosha antibody (ab58589)IHC image of ab58589 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab58589, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
