Anti-Drosha antibody (ab12286)
Key features and details
- Rabbit polyclonal to Drosha
- Suitable for: WB, ICC
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Drosha antibody
See all Drosha primary antibodies -
Description
Rabbit polyclonal to Drosha -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human Drosha aa 1-100 (internal sequence).
(Peptide available asab12307) -
General notes
Three isoforms of 159, 156 and 143 kDa.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab12286 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB (4) Use a concentration of 1 µg/ml. Predicted molecular weight: 159 kDa.ICC (1) Use a concentration of 1 µg/ml.Notes WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 159 kDa.ICC
Use a concentration of 1 µg/ml.Target
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Function
Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Contains 1 DRBM (double-stranded RNA-binding) domain.
Contains 2 RNase III domains. -
Domain
The 2 RNase III domains form an intramolecular dimer where the domain 1 cuts the 3'strand while the domain 2 cleaves the 5'strand of pri-miRNAs, independently of each other. -
Cellular localization
Nucleus. Nucleus > nucleolus. A fraction is translocated to the nucleolus during the S phase of the cell cycle. Localized in GW bodies (GWBs), also known as P-bodies. - Information by UniProt
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Database links
- Entrez Gene: 29102 Human
- Entrez Gene: 14000 Mouse
- Omim: 608828 Human
- SwissProt: Q9NRR4 Human
- SwissProt: Q5HZJ0 Mouse
- Unigene: 97997 Human
- Unigene: 293142 Mouse
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Alternative names
- DROSHA antibody
- Drosha double stranded RNA specific endoribonuclease antibody
- Drosha ribonuclease type III antibody
see all
Images
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Immunocytochemistry - Anti-Drosha antibody - ChIP Grade (ab12286)This image is courtesy of Ahmad M. Khalil and Daniel J. Driscoll, University of Florida College of Medicine Genetics Institute.Human female amniocytes immunostained with ab12286 Drosha (FITC) (1/25 dilution). The DNA is labelled red with propidium iodide. This image was submitted as part of a review by Ahmad Khalil.
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Western blot - Anti-Drosha antibody - ChIP Grade (ab12286)All lanes : Anti-Drosha antibody (ab12286) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 159 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted? -
Immunocytochemistry - Anti-Drosha antibody (ab12286)ab12286 at a 1/25 dilution staining human HeLa cells by immunocytochemistry. The antibody was incubated with the cells for 1 hour and then detected using a Cy3 conjugated donkey anti-rabbit polyclonal antibody.
This image is courtesy of an Abreview submitted on 7 February 2006.
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Western blot - Anti-Drosha antibody - ChIP Grade (ab12286)All lanes : Anti-Drosha antibody (ab12286) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Nuclear Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 159 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Additional bands at: 115 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
Immunocytochemistry - Anti-Drosha antibody (ab12286)ICC/IF image of ab12286 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12286, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunocytochemistry - Anti-Drosha antibody (ab12286)ICC/IF image of ab12286 stained Hela cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12886, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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Datasheet download
References (83)
ab12286 has been referenced in 83 publications.
- Zhang L et al. RNA helicase p68 inhibits the transcription and post-transcription of Pkd1 in ADPKD. Theranostics 10:8281-8297 (2020). PubMed: 32724471
- Fóthi Á et al. Tissue-specific and transcription-dependent mechanisms regulate primary microRNA processing efficiency of the human chromosome 19 MicroRNA cluster. RNA Biol N/A:1-11 (2020). PubMed: 33052778
- Milon A et al. Effect of estrogen-related receptor silencing on miRNA protein machinery expression, global methylation, and deacetylation in bank vole (Myodes glareolus) and mouse tumor Leydig cells. Theriogenology 139:178-190 (2019). PubMed: 31421412
- Pan D et al. Herpes Simplex Virus 1 Lytic Infection Blocks MicroRNA (miRNA) Biogenesis at the Stage of Nuclear Export of Pre-miRNAs. MBio 10:N/A (2019). PubMed: 30755517
- Zhang J et al. Excessive miR-25-3p maturation via N6-methyladenosine stimulated by cigarette smoke promotes pancreatic cancer progression. Nat Commun 10:1858 (2019). PubMed: 31015415
Images
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Immunocytochemistry - Anti-Drosha antibody - ChIP Grade (ab12286) This image is courtesy of Ahmad M. Khalil and Daniel J. Driscoll, University of Florida College of Medicine Genetics Institute.Human female amniocytes immunostained with ab12286 Drosha (FITC) (1/25 dilution). The DNA is labelled red with propidium iodide. This image was submitted as part of a review by Ahmad Khalil.
-
Western blot - Anti-Drosha antibody - ChIP Grade (ab12286)All lanes : Anti-Drosha antibody (ab12286) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 159 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
-
Immunocytochemistry/ Immunofluorescence - Anti-Drosha antibody - ChIP Grade (ab12286)
ab12286 at a 1/25 dilution staining human HeLa cells by immunocytochemistry. The antibody was incubated with the cells for 1 hour and then detected using a Cy3 conjugated donkey anti-rabbit polyclonal antibody.
This image is courtesy of an Abreview submitted on 7 February 2006.
-
Western blot - Anti-Drosha antibody - ChIP Grade (ab12286)All lanes : Anti-Drosha antibody (ab12286) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Nuclear Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 159 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Additional bands at: 115 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
Immunocytochemistry/ Immunofluorescence - Anti-Drosha antibody - ChIP Grade (ab12286)ICC/IF image of ab12286 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12286, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
-
Immunocytochemistry/ Immunofluorescence - Anti-Drosha antibody - ChIP Grade (ab12286)ICC/IF image of ab12286 stained Hela cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12886, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
