Anti-DKK1 antibody (ab61275)
Key features and details
- Mouse monoclonal to DKK1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-DKK1 antibody
See all DKK1 primary antibodies -
Description
Mouse monoclonal to DKK1 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Horse, Cow, Chimpanzee, Rhesus monkey
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Immunogen
Recombinant fragment with tag: QPYPCAEDEE CGTDEYCASP TRGGDAGVQI CLACRKRRKR CMRHAMCCPG NYCKNGICVS SDQNHFRGEI EETITESFGN DHSTLDGYSR RTTLSSKMYH , corresponding to amino acids 81-181 of Human DKK1
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Positive control
- HeLa cell lysate.
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General notes
This product was changed from ascites to tissue culture supernatant on 05 Feb 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: PBS -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-DKK1 antibody (ab61275)Anti-DKK1 antibody (ab61275) at 1 µg/ml + HeLa cell lysate at 25 µg
Predicted band size: 29 kDa
Observed band size: 29 kDa
Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.This image was generated using a version of the antibody produced in ascites.
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Immunocytochemistry/ Immunofluorescence - Anti-DKK1 antibody (ab61275)ICC/IF image of ab61275 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61275, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image was generated using a version of the antibody produced in ascites.
