Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DKK1 antibody (ab93017)

Key features and details

  • Rabbit polyclonal to DKK1
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-DKK1 antibody
    See all DKK1 primary antibodies

  • Description

    Rabbit polyclonal to DKK1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Pig

  • Immunogen

    Synthetic peptide corresponding to Human DKK1 aa 150-250 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab101729)

  • Positive control

    • Recombinant human DKK1 protein (ab82115) can be used as a positive control in WB. This antibody gave a positive signal in the following whole cell lysates: HeLa; Saos 2; HepG2.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab93017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 29 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.

  • Tissue specificity

    Placenta.

  • Sequence similarities

    Belongs to the dickkopf family.

  • Domain

    The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Dickkopf 1 antibody
    • Dickkopf 1 homolog antibody
    • Dickkopf 1 like antibody
    • Dickkopf homolog 1 antibody
    • Dickkopf like protein 1 antibody
    • Dickkopf related protein 1 antibody
    • Dickkopf WNT signaling pathway inhibitor 1 antibody
    • Dickkopf-1 antibody
    • Dickkopf-related protein 1 antibody
    • DKK 1 antibody
    • Dkk-1 antibody
    • Dkk1 antibody
    • DKK1_HUMAN antibody
    • hDkk 1 antibody
    • hDkk-1 antibody
    • SK antibody
    see all

Images

  • Western blot - Anti-DKK1 antibody (ab93017)
    Western blot - Anti-DKK1 antibody (ab93017)
    All lanes : Anti-DKK1 antibody (ab93017) at 1 µg/ml

    Lane 1 : PC-3 whole cell lysate (ab3954)
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size: 29 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-DKK1 antibody (ab93017)
    Western blot - Anti-DKK1 antibody (ab93017)
    All lanes : Anti-DKK1 antibody (ab93017) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 29 kDa
    Observed band size: 38 kDa why is the actual band size different from the predicted?
    Additional bands at: 200 kDa, 21 kDa, 29 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes


    Please note that a band of similar size is reported in the literature (PMID 10383463).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKK1 antibody (ab93017)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKK1 antibody (ab93017)
    IHC image of DKK1 staining in human placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab93017, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

References (2)

Publishing research using ab93017? Please let us know so that we can cite the reference in this datasheet.

ab93017 has been referenced in 2 publications.

  • Liao S  et al. Chemoprevention of elite tea variety CFT-1 rich in EGCG against chemically induced liver cancer in rats. Food Sci Nutr 7:2647-2665 (2019). PubMed: 31428352
  • Ma L  et al. The association between parathyroid hormone 1-34 and the Wnt/ß-catenin signaling pathway in a rat model of osteoarthritis. Mol Med Rep 16:8799-8807 (2017). PubMed: 29039525

Images

  • Western blot - Anti-DKK1 antibody (ab93017)
    Western blot - Anti-DKK1 antibody (ab93017)
    All lanes : Anti-DKK1 antibody (ab93017) at 1 µg/ml

    Lane 1 : PC-3 whole cell lysate (ab3954)
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size: 29 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-DKK1 antibody (ab93017)
    Western blot - Anti-DKK1 antibody (ab93017)
    All lanes : Anti-DKK1 antibody (ab93017) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 29 kDa
    Observed band size: 38 kDa why is the actual band size different from the predicted?
    Additional bands at: 200 kDa, 21 kDa, 29 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes


    Please note that a band of similar size is reported in the literature (PMID 10383463).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKK1 antibody (ab93017)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKK1 antibody (ab93017)
    IHC image of DKK1 staining in human placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab93017, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

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