Antibodies, Proteins, Kits and Reagents for Life Science

274 732 ₸ Product size

100 µg 274 732 ₸

Order now and get it on Thursday February 25, 2021

Key features and details

Rabbit polyclonal to DKK1
Suitable for: WB, IHC-P
Reacts with: Human
Isotype: IgG

Product name

Anti-DKK1 antibody
See all DKK1 primary antibodies
Description

Rabbit polyclonal to DKK1
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Pmore details
Species reactivity

Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Pig
Immunogen

Synthetic peptide corresponding to Human DKK1 aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available as ab101729)
Positive control
- Recombinant human DKK1 protein (ab82115) can be used as a positive control in WB. This antibody gave a positive signal in the following whole cell lysates: HeLa; Saos 2; HepG2.
General notes

Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS

Batches of this product that have a concentration
Concentration information loading...
Purity

Immunogen affinity purified
Clonality

Polyclonal
Isotype

IgG
Research areas

Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
Positive Controls
- Recombinant human DKK1 protein (ab82115)
- Recombinant Human DKK1 protein (ab87416)
Recombinant Protein
- Recombinant human DKK1 protein (ab155623)

Our Abpromise guarantee covers the use of ab93017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 29 kDa).
IHC-P		Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Function

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
Tissue specificity

Placenta.
Sequence similarities

Belongs to the dickkopf family.
Domain

The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
Cellular localization

Secreted.
Target information above from: UniProt accession O94907 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 22943 Human
- Entrez Gene: 13380 Mouse
- Entrez Gene: 100157640 Pig
- Entrez Gene: 293897 Rat
- Omim: 605189 Human
- SwissProt: O94907 Human
- SwissProt: O54908 Mouse
- Unigene: 40499 Human
- Unigene: 214717 Mouse
see all
Alternative names
- Dickkopf 1 antibody
- Dickkopf 1 homolog antibody
- Dickkopf 1 like antibody
- Dickkopf homolog 1 antibody
- Dickkopf like protein 1 antibody
- Dickkopf related protein 1 antibody
- Dickkopf WNT signaling pathway inhibitor 1 antibody
- Dickkopf-1 antibody
- Dickkopf-related protein 1 antibody
- DKK 1 antibody
- Dkk-1 antibody
- Dkk1 antibody
- DKK1_HUMAN antibody
- hDkk 1 antibody
- hDkk-1 antibody
- SK antibody
see all

Western blot - Anti-DKK1 antibody (ab93017)

All lanes : Anti-DKK1 antibody (ab93017) at 1 µg/ml

Lane 1 : PC-3 whole cell lysate (ab3954)
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 29 kDa
Observed band size: 38 kDa
why is the actual band size different from the predicted?
Western blot - Anti-DKK1 antibody (ab93017)

All lanes : Anti-DKK1 antibody (ab93017) at 1 µg/ml

Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 29 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Additional bands at: 200 kDa, 21 kDa, 29 kDa. We are unsure as to the identity of these extra bands.

Exposure time: 20 minutes

Please note that a band of similar size is reported in the literature (PMID 10383463).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKK1 antibody (ab93017)

IHC image of DKK1 staining in human placenta formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab93017, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Western blot protocols
Immunohistochemistry protocols

Click here to view the general protocols

Datasheet

Publishing research using ab93017? Please let us know so that we can cite the reference in this datasheet.

ab93017 has been referenced in 2 publications.

Liao S et al. Chemoprevention of elite tea variety CFT-1 rich in EGCG against chemically induced liver cancer in rats. Food Sci Nutr 7:2647-2665 (2019). PubMed: 31428352
Ma L et al. The association between parathyroid hormone 1-34 and the Wnt/ß-catenin signaling pathway in a rat model of osteoarthritis. Mol Med Rep 16:8799-8807 (2017). PubMed: 29039525

Western blot - Anti-DKK1 antibody (ab93017)

All lanes : Anti-DKK1 antibody (ab93017) at 1 µg/ml

Lane 1 : PC-3 whole cell lysate (ab3954)
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Predicted band size: 29 kDa
Observed band size: 38 kDa
why is the actual band size different from the predicted?
Western blot - Anti-DKK1 antibody (ab93017)

All lanes : Anti-DKK1 antibody (ab93017) at 1 µg/ml

Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 29 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Additional bands at: 200 kDa, 21 kDa, 29 kDa. We are unsure as to the identity of these extra bands.

Exposure time: 20 minutes

Please note that a band of similar size is reported in the literature (PMID 10383463).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKK1 antibody (ab93017)

IHC image of DKK1 staining in human placenta formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab93017, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.