Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DKK1 antibody [EPR4759] (ab109416)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4759] to DKK1
  • Suitable for: WB, IHC-P
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-DKK1 antibody [EPR4759]
    See all DKK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR4759] to DKK1

  • Host species

    Rabbit

  • Specificity

    Although our internal data shows that ab109416 is not suitable to detect DKK1 in rats, ab109416 has been referenced in PMID 29299122 and 25469240 to work with rat derived cells. Please contact technical@abcam.com for further information.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293, Caco-2, A549, PC-3, NCI-H460, DU145 and SK-OV-3 whole cell lysates IHC-P: Human placenta and liver tissue

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-DKK1 antibody [EPR4759] (ab109416)
    Western blot - Anti-DKK1 antibody [EPR4759] (ab109416)
    All lanes : Anti-DKK1 antibody [EPR4759] (ab109416) at 1/10000 dilution

    Lane 1 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 29 kDa
    Observed band size: 29-40 kDa
    why is the actual band size different from the predicted?


    Exposure time: 180 seconds


    Blocking/Diluting buffer and concentration 5% NFDM/TBST.

    HEK-293 and 293T cell are weak expressed cell lines for DKK1 protein (PMID: 18502762,  PMID: 15378020).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKK1 antibody [EPR4759] (ab109416)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKK1 antibody [EPR4759] (ab109416)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling DKK1 with Purified ab109416 at 1:800 dilution (1.04 µg/ml). Heat mediated antigen retrieval was performed using using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
  • Western blot - Anti-DKK1 antibody [EPR4759] (ab109416)
    Western blot - Anti-DKK1 antibody [EPR4759] (ab109416)
    Anti-DKK1 antibody [EPR4759] (ab109416) at 1/1000 dilution + His-Tagged human DKK1 (aa 32 to 266) recombinant protein, 100ng

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 29 kDa



    Blocking/Diluting buffer and concentration 5% NFDM /TBST

    Exposure time: 5.5 seconds

     

  • Western blot - Anti-DKK1 antibody [EPR4759] (ab109416)
    Western blot - Anti-DKK1 antibody [EPR4759] (ab109416)
    All lanes : Anti-DKK1 antibody [EPR4759] (ab109416) at 1/2000 dilution (purified)

    Lane 1 : A549 (Human lung carcinoma epithelial cell) whole cell lysates
    Lane 2 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates
    Lane 3 : NCI-H460 (Human Large Cell Lung Cancer epithelial cell) whole cell lysates
    Lane 4 : DU145 (Human prostate carcinoma epithelial cell) whole cell lysates
    Lane 5 : SK-OV-3 (Human ovarian cancer epithelial cell) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 29 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

    The smear bands detected by ab109416 are similar to those reported by the papers (PMID: 18546262, 28090290, 24008318), which should be due to the glycosylation of DKK1. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKK1 antibody [EPR4759] (ab109416)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKK1 antibody [EPR4759] (ab109416)

    Immunohistochemical analysis of paraffin-embedded Human placenta tissue using 1/100 unpurified ab109416.

  • Anti-DKK1 antibody [EPR4759] (ab109416)
    Anti-DKK1 antibody [EPR4759] (ab109416)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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