Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4759] to DKK1
Suitable for: WB, IHC-P
Reacts with: Human, Recombinant fragment

Product name

Anti-DKK1 antibody [EPR4759]
See all DKK1 primary antibodies
Description

Rabbit monoclonal [EPR4759] to DKK1
Host species

Rabbit
Specificity

Although our internal data shows that ab109416 is not suitable to detect DKK1 in rats, ab109416 has been referenced in PMID 29299122 and 25469240 to work with rat derived cells. Please contact technical@abcam.com for further information.
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

Recombinant fragment

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK-293, Caco-2, A549, PC-3, NCI-H460, DU145 and SK-OV-3 whole cell lysates IHC-P: Human placenta and liver tissue
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IHC-P	Human
WB	Human Recombinant fragment

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4759
Isotype

IgG
Research areas

Western blot - Anti-DKK1 antibody [EPR4759] (ab109416)

All lanes : Anti-DKK1 antibody [EPR4759] (ab109416) at 1/10000 dilution

Lane 1 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 29 kDa
Observed band size: 29-40 kDa
why is the actual band size different from the predicted?

Exposure time: 180 seconds

Blocking/Diluting buffer and concentration 5% NFDM/TBST.

HEK-293 and 293T cell are weak expressed cell lines for DKK1 protein (PMID: 18502762, PMID: 15378020).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKK1 antibody [EPR4759] (ab109416)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling DKK1 with Purified ab109416 at 1:800 dilution (1.04 µg/ml). Heat mediated antigen retrieval was performed using using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
Western blot - Anti-DKK1 antibody [EPR4759] (ab109416)

Anti-DKK1 antibody [EPR4759] (ab109416) at 1/1000 dilution + His-Tagged human DKK1 (aa 32 to 266) recombinant protein, 100ng

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 29 kDa

Blocking/Diluting buffer and concentration 5% NFDM /TBST

Exposure time: 5.5 seconds
Western blot - Anti-DKK1 antibody [EPR4759] (ab109416)

All lanes : Anti-DKK1 antibody [EPR4759] (ab109416) at 1/2000 dilution (purified)

Lane 1 : A549 (Human lung carcinoma epithelial cell) whole cell lysates
Lane 2 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates
Lane 3 : NCI-H460 (Human Large Cell Lung Cancer epithelial cell) whole cell lysates
Lane 4 : DU145 (Human prostate carcinoma epithelial cell) whole cell lysates
Lane 5 : SK-OV-3 (Human ovarian cancer epithelial cell) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 29 kDa

Blocking and diluting buffer: 5% NFDM/TBST.

The smear bands detected by ab109416 are similar to those reported by the papers (PMID: 18546262, 28090290, 24008318), which should be due to the glycosylation of DKK1.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKK1 antibody [EPR4759] (ab109416)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue using 1/100 unpurified ab109416.
Anti-DKK1 antibody [EPR4759] (ab109416)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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