Anti-HIRA/HIR antibody (ab20655)
Key features and details
- Rabbit polyclonal to HIRA/HIR
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HIRA/HIR antibody
See all HIRA/HIR primary antibodies -
Description
Rabbit polyclonal to HIRA/HIR -
Host species
Rabbit -
Specificity
ab20655 is able to recognise a band at 115kDa corresponding to the long isoform of HIRA/HIR and a band at 89kDa, corresponding to the short isoform of HIRA/HIR in HepG2 nuclear extract.
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Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
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General notes
This product was previously labelled as HIRA
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab20655 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatDogApplication Abreviews Notes WB (2) Use a concentration of 1 µg/ml. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).Detects a band of approximately 89 kDa, corresponding to the short isoform of HIRA/HIR.
Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).Detects a band of approximately 89 kDa, corresponding to the short isoform of HIRA/HIR.
Target
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Function
Cooperates with ASF1A to promote replication-independent chromatin assembly. Required for the periodic repression of histone gene transcription during the cell cycle. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit. -
Tissue specificity
Expressed at high levels in kidney, pancreas and skeletal muscle and at lower levels in brain, heart, liver, lung, and placenta. -
Sequence similarities
Belongs to the WD repeat HIR1 family.
Contains 8 WD repeats. -
Developmental stage
Expressed during embryogenesis. -
Post-translational
modificationsSumoylated.
Phosphorylated by CDK2/CCNA1 and CDK2/CCNE1 on Thr-555 in vitro. Also phosphorylated on Thr-555 and Ser-687 in vivo. -
Cellular localization
Nucleus. Nucleus > PML body. Primarily, though not exclusively, localized to the nucleus. Localizes to PML bodies immediately prior to onset of senescence. - Information by UniProt
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Database links
- Entrez Gene: 7290 Human
- Entrez Gene: 15260 Mouse
- Entrez Gene: 363849 Rat
- Omim: 600237 Human
- SwissProt: P54198 Human
- SwissProt: Q61666 Mouse
- Unigene: 474206 Human
- Unigene: 15694 Mouse
see all -
Alternative names
- DGCR1 antibody
- DiGeorge critical region gene 1 antibody
- HIR antibody
see all
Images
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Western blot - Anti-HIRA/HIR antibody (ab20655)ab20655 at 10 µg + HepG2 nuclear extract lysate at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 89 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted?
Additional bands at: 89 kDa (possible isoform)ab20655 is able to recognise a band at 115kDa corresponding to the long isoform of HIRA/HIR and a band at 89kDa, corresponding to the short isoform of HIRA/HIR.
Protocols
Datasheets and documents
References (8)
ab20655 has been referenced in 8 publications.
- Saavedra F et al. JMJD1B, a novel player in histone H3 and H4 processing to ensure genome stability. Epigenetics Chromatin 13:6 (2020). PubMed: 32070414
- Zhang Q et al. Role of lncRNA uc.457 in the differentiation and maturation of cardiomyocytes. Mol Med Rep 19:4927-4934 (2019). PubMed: 30957182
- Cohen C et al. Promyelocytic leukemia (PML) nuclear bodies (NBs) induce latent/quiescent HSV-1 genomes chromatinization through a PML NB/Histone H3.3/H3.3 Chaperone Axis. PLoS Pathog 14:e1007313 (2018). PubMed: 30235352
- Rivera C et al. Methylation of histone H3 lysine 9 occurs during translation. Nucleic Acids Res 43:9097-106 (2015). PubMed: 26405197
- Majumder A et al. Histone Chaperone HIRA in Regulation of Transcription Factor RUNX1. J Biol Chem 290:13053-63 (2015). PubMed: 25847244
- O'Sullivan RJ et al. Rapid induction of alternative lengthening of telomeres by depletion of the histone chaperone ASF1. Nat Struct Mol Biol 21:167-74 (2014). WB ; Human . PubMed: 24413054
- Lewis PW et al. Daxx is an H3.3-specific histone chaperone and cooperates with ATRX in replication-independent chromatin assembly at telomeres. Proc Natl Acad Sci U S A 107:14075-80 (2010). ChIP ; Mouse . PubMed: 20651253
- Dutta D et al. Regulation of angiogenesis by histone chaperone HIRA-mediated incorporation of lysine 56-acetylated histone H3.3 at chromatin domains of endothelial genes. J Biol Chem 285:41567-77 (2010). WB ; Mouse . PubMed: 21041298
Images
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Western blot - Anti-HIRA/HIR antibody (ab20655)ab20655 at 10 µg + HepG2 nuclear extract lysate at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 89 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted?
Additional bands at: 89 kDa (possible isoform)ab20655 is able to recognise a band at 115kDa corresponding to the long isoform of HIRA/HIR and a band at 89kDa, corresponding to the short isoform of HIRA/HIR.
