Anti-P5CS antibody (ab223713)
Key features and details
- Rabbit polyclonal to P5CS
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-P5CS antibody
See all P5CS primary antibodies -
Description
Rabbit polyclonal to P5CS -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Orangutan
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Immunogen
Recombinant fragment corresponding to Human P5CS aa 50-250.
Database link: P54886 -
Positive control
- WB: NIH/3T3, NBT-II and U-251 cell lysates. IHC-P: Human colon tissue. ICC/IF: U-2 OS cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-P5CS antibody (ab223713)All lanes : Anti-P5CS antibody (ab223713) at 1/100 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) cell lysate
Predicted band size: 87 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P5CS antibody (ab223713)Paraffin embedded sections of human colon were stained for P5CS with ab223713 at 1/20 dilution in immunohistochemical analysis.
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Western blot - Anti-P5CS antibody (ab223713)All lanes : Anti-P5CS antibody (ab223713) at 1/20 dilution
Lane 1 : Caco-2 cell lysate transfected with control siRNA
Lane 2 : Caco-2 cell lysate transfected with target specific siRNA probe #1
Lane 3 : Caco-2 cell lysate transfected with target specific siRNA probe #2
Predicted band size: 87 kDa
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Western blot - Anti-P5CS antibody (ab223713)All lanes : Anti-P5CS antibody (ab223713) at 1/100 dilution
Lane 1 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate
Lane 2 : NBT-II (rat bladder tumor cell line) cell lysate
Predicted band size: 87 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-P5CS antibody (ab223713)PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for P5CS (green) using ab223713 at 4 µg/ml in ICC/IF.
