Fructose-1,6-Bisphosphatase Activity Assay Kit (Colorimetric) (ab273329)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
Fructose-1,6-Bisphosphatase Activity Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Enzyme activity (quantitative) -
Assay duration
Multiple steps standard assay -
Product overview
Fructose-1,6-Bisphosphatase Activity Assay Kit (Colorimetric) (ab273329) provides a quick, reliable test that allows the measurement of Fructose-1,6-Bisphosphatase (FBP) enzymatic activity in various samples. In this assay, FBP hydrolyzes Fructose-1,6-Bisphosphate into Fructose-6-Phosphate (F6P). F6P is used in an enzyme coupled reaction which reduces a chromophore forming a product with a stable signal that can measured at OD = 450 nm.
The assay is simple, sensitive and can detect lower than 100 µU of FBP in variety of samples.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests F6P Standard (Lyophilized) 1 vial FBP Assay Buffer 1 x 25ml FBP Converter (Lyophilized) 1 vial FBP Developer (Lyophilized) 1 vial FBP Positive Control (Lyophilized) 1 vial FBP Probe (Lyophilized) 1 vial FBP Substrate (Lyophilized) 1 vial -
Research areas
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Cellular localization
FBP2: Cell junction. Cytoplasm. Nucleus. Cytoplasm, myofibril, sarcomere, Z line. In neonatal cardiomyocytes, distributed throughout the cytosol, accumulating in the intercalated disks which occur at the Z line of cardiomyocytes and connect adjacent cells, and also located in the nucleus; dissociates from the Z line following an increase in cytosolic Ca(2+) concentration (By similarity). In muscle precursor cells, localizes predominantly to the nucleus and to a lesser extent to the cytoplasm at the proliferative phase, while mainly localizing to the cytoplasm at the differentiation phase (By similarity). Colocalizes with ALDOA and alpha-actinin on both sides of the Z line of skeletal muscle; dissociates rapidly from the Z line following an increase in cytosolic Ca(2+) concentration.
Images
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Example data
Example data.
F6P standard curve.
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Example dataExample data.
Kinetic measurement of Fructose-1,6-Biosphophatase activity from various samples.
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Example dataExample data.
Specific FBP Activity was calculated in lysates prepared from rat liver (3.2µg protein), rat kidney (8µg protein) and HeLa lysate (4µg protein).