Human ABCA1 Antibody Pair - BSA and Azide free (ab253546)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human
Overview
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Product name
Human ABCA1 Antibody Pair - BSA and Azide free -
Assay type
ELISA set -
Range
39 pg/ml - 10000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
- Human ABCA1 Antibody Pair is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify Human ABCA1 in sandwich ELISAs and many other pair-based applications.
- The pair can be used in variety of assays and platforms including but not limited to:
- - Sandwich ELISA
- - FRET/TR-FRET/HTR
- - Meso Scale Discovery® (MSD®)
- - Luminex® and bead-based assays
- - AlphaLISA®/AlphaScreen®
- - DELFIA® immunoassays
- - Simoa® and Single Molecule Counting (SMC™) immunoassays
- - Multiplex
- Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:
- - Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
- - Concentration of ~1 mg/ml as measured by the protein A280 method.
- Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
- We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.
- Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.
- Please note:
- The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
- The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
- We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.
- Antibody properties:
- Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg
- Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg
- Concentration: ~1 mg/ml
- Storage buffer: 100% PBS
- Form: Liquid
- Isotype: IgG
- Recombinant monoclonal antibodies offer several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
- For more information see here.
- Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
- Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
- Luminex is a trademark of Luminex Corporation, registered in the US and other countries.
- AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
- Simoa is a registered trademark of Quanterix, Inc.
- SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components Identifier 10 x 96 tests Human ABCA1 Capture Antibody (unconjugated) — Human ABCA1 Detector Antibody (unconjugated) — -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
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Function
cAMP-dependent and sulfonylurea-sensitive anion transporter. Key gatekeeper influencing intracellular cholesterol transport. -
Tissue specificity
Widely expressed, but most abundant in macrophages. -
Involvement in disease
Defects in ABCA1 are a cause of high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by absence of high density lipoprotein (HDL) cholesterol from plasma, accumulation of cholesteryl esters, premature coronary artery disease (CAD), hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness.
Defects in ABCA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). HDLD2 is inherited as autosomal dominant trait. It is characterized by moderately low HDL cholesterol, predilection toward premature coronary artery disease (CAD) and a reduction in cellular cholesterol efflux. -
Sequence similarities
Belongs to the ABC transporter superfamily. ABCA family.
Contains 2 ABC transporter domains. -
Domain
Multifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain. -
Post-translational
modificationsPhosphorylation on Ser-2054 regulates phospholipid efflux.
Palmitoylation by DHHC8 is essential for membrane localization. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- ABC 1
- ABC Transporter 1
- ABC-1
see all -
Database links
- Entrez Gene: 19 Human
- Omim: 600046 Human
- SwissProt: O95477 Human
- Unigene: 429294 Human
Images
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Sandwich ELISA - Antibody Pair - BSA and Azide Free (ab253546)To learn more about the advantages of recombinant antibodies see here.