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Neuroscience Cell Type Marker Glia marker Astrocyte marker

Alexa Fluor® 488 Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (ab200771)

Price and availability

334 369 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Alexa Fluor® 488 Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (ab200771)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EPR9724(B)] to Aldolase + Aldolase B + Aldolase C
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

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Overview

  • Product name

    Alexa Fluor® 488 Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)]
  • Description

    Alexa Fluor® 488 Rabbit monoclonal [EPR9724(B)] to Aldolase + Aldolase B + Aldolase C
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human Aldolase.
    Database link: P04075

  • Positive control

    • Flow Cyt: MCF7 cells. WB: His-tagged human Aldolase A, B and C full-length recombinant protein.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR9724(B)
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Astrocyte marker
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia

Images

  • Western blot - Alexa Fluor® 488 Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (ab200771)
    Western blot - Alexa Fluor® 488 Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (ab200771)
    All lanes : Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (ab169544) at 1/1000 dilution

    Lane 1 : His-tagged human Aldolase A full-length recombinant protein
    Lane 2 : His-tagged human Aldolase B full-length recombinant protein
    Lane 3 : His-tagged human Aldolase C full-length recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)


    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab169544).

  • Flow Cytometry - Alexa Fluor® 488 Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (ab200771)
    Flow Cytometry - Alexa Fluor® 488 Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (ab200771)

    Overlay histogram showing MCF7 cells stained with ab200771 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab200771, 1/100 dilution) for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Alexa Fluor® 488 Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (ab200771)
    Alexa Fluor® 488 Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (ab200771)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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