Anti-ABCG1 antibody [EP1366Y] - BSA and Azide free (ab218528)
![Anti-ABCG1 antibody [EP1366Y] - BSA and Azide free (ab218528)](https://www.abcam.com/ps/products/218/ab218528/Images/ab218528-330282-anti-abcg1-antibody-ep1366y-immunofluorescence.jpg "Anti-ABCG1 antibody [EP1366Y] - BSA and Azide free (ab218528)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1366Y] to ABCG1 - BSA and Azide free
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Cow, Human
Overview
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Product name
Anti-ABCG1 antibody [EP1366Y] - BSA and Azide free
See all ABCG1 primary antibodies -
Description
Rabbit monoclonal [EP1366Y] to ABCG1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P Human
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: HUVEC cell lysate.
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General notes
Ab218528 is the carrier-free version of ab52617. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab218528 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1366Y -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ABCG1 antibody [EP1366Y] - BSA and Azide free (ab218528)
ab52617 staining ABCG1 in HUVEC (Human umbilical vein endothelial cell line) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab195889 was used as a counterstain for primary antibody ab52617 at 1/200. DAPI was used as a nuclear counterstain and PBS as a negative control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52617).
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![Anti-ABCG1 antibody [EP1366Y] - BSA and Azide free (ab218528)](https://www.abcam.com/ps/products/218/ab218528/Images/ab218528-3-benefits-of-recombinant-antibodies.png)
Anti-ABCG1 antibody [EP1366Y] - BSA and Azide free (ab218528)
