Recombinant human KDM3A / JHDM2A protein (ab196411)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 27% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human KDM3A / JHDM2A protein -
Biological activity
0.3 pmole/min/μg: 10µl reaction mix containing assay buffer with 20 mM Tris (pH 7.4), 150 mM NaCl, 500 µM α-ketoglutarate, 25 µM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and ab196411 (20 – 100 ng) added to the wells. Add antibody against mono-methylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.
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Purity
>= 27 % SDS-PAGE.
ab196411 is greater or equal to 27% pure as assessed by SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
VLTLGESWPVLVGRRFLSLSAADGSDGSHDSWDVERVAEWPWLSGTIRAV SHTDVTKKDLKVCVEFDGESWRKRRWIEVYSLLRRAFLVEHNLVLAERKS PEISERIVQWPAITYKPLLDKAGLGSITSVRFLGDQQRVFLSKDLLKPIQ DVNSLRLSLTDNQIVSKEFQALIVKHLDESHLLKGDKNLVGSEVKIYSLD PSTQWFSATVINGNPASKTLQVNCEEIPALKIVDPSLIHVEVVHDNLVTC GNSARIGAVKRKSSENNGTLVSKQAKSCSEASPSMCPVQSVPTTVFKEIL LGCTAATPPSKDPRQQSTPQAANSPPNLGAKIPQGCHKQSLPEEISSCLN TKSEALRTKPDVCKAGLLSKSSQIGTGDLKILTEPKGSCTQPKTNTDQEN RLESVPQALTGLPKECLPTKASSKAELEIANPPELQKHLEHAPSPSDVSN APEVKAGVNSDSPNNCSGKKVEPSALACRSQNLKESSVKVDNESCCSRSN NKIQNAPSRKSVLTDPAKLKKLQQSGEAFVQDDSCVNIVAQLPKCRECRL DSLRKDKEQQKDSPVFCRFFHFRRLQFNKHGVLRVEGFLTPNKYDNEAIG LWLPLTKNVVGIDLDTAKYILANIGDHFCQMVISEKEAMSTIEPHRQVAW KRAVKGVREMCDVCDTTIFNLHWVCPRCGFGVCVDCYRMKRKNCQQGAAY KTFSWLKCVKSQIHEPENLMPTQIIPGKALYDVGDIVHSVRAKWGIKANC PCSNRQFKLFSKPASKEDLKQTSLAGEKPTLGAVLQQNPSVLEPAAVGGE AASKPAGSMKPACPASTSPLNWLADLTSGNVNKENKEKQPTMPILKNEIK CLPPLPPLSKSSTVLHTFNSTILTPVSNNNSGFLRNLLNSSTGKTENGLK NTPKILDDIFASLVQNKTTSDLSKRPQGLTIKPSILGFDTPHYWLCDNRL LCLQDPNNKSNWNVFRECWKQGQPVMVSGVHHKLNSELWKPESFRKEFGE QEVDLVNCRTNEIITGATVGDFWDGFEDVPNRLKNEKEPMVLKLKDWPPG EDFRDMMPSRFDDLMANIPLPEYTRRDGKLNLASRLPNYFVRPDLGPKMY NAYGLITPEDRKYGTTNLHLDVSDAANVMVYVGIPKGQCEQEEEVLKTIQ DGDSDELTIKRFIEGKEKPGALWHIYAAKDTEKIREFLKKVSEEQGQENP ADHDPIHDQSWYLDRSLRKRLHQEYGVQGWAIVQFLGDVVFIPAGAPHQV HNLYSCIKVAEDFVSPEHVKHCFWLTQEFRYLSQTHTNHEDKLQVKNVIY HAVKDAVAMLKASESSFGKP -
Predicted molecular weight
148 kDa including tags -
Amino acids
2 to 1321 -
Tags
DDDDK tag N-Terminus
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.71% Tris HCl, 0.72% Sodium chloride, 0.02% Potassium chloride, 10% Glycerol (glycerin, glycerine)
contains 90ng/ml DDDDK peptideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant human KDM3A / JHDM2A protein (ab196411)
4-20% SDS-PAGE analysis of ab196411 at 1.6 µg stained with Coomassie Blue.
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Functional Studies - Recombinant human KDM3A / JHDM2A protein (ab196411)Example of specific activity of ab196411
