Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Tuesday March 02, 2021

Anti-PON2 antibody [EPR15295-82] (ab183710)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15295-82] to PON2
Suitable for: WB, IHC-P, IP
Knockout validated
Reacts with: Mouse, Human

Product name

Anti-PON2 antibody [EPR15295-82]
See all PON2 primary antibodies
Description

Rabbit monoclonal [EPR15295-82] to PON2
Host species

Rabbit
Specificity

Based on the sequence analysis, ab183710 recognizes three isoforms with the predicted MWts of 39KDa, 39KDa and 38KDa, respectively.

Tested Applications & Species

Application	Species
IHC-P	Mouse Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, HepG2, A431 and U87-MG cell lysates. IHC-P: Human and Mouse kidney tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15295-82
Isotype

IgG
Research areas

Western blot - Anti-PON2 antibody [EPR15295-82] (ab183710)

All lanes : Anti-PON2 antibody [EPR15295-82] (ab183710) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PON2 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 39 kDa
Observed band size: 39,41 kDa
why is the actual band size different from the predicted?

Lanes 1-3: Merged signal (red and green). Green - ab183710 observed at 39,41 kDa. Red - loading control ab7291 observed at 50 kDa.

ab183710 Anti-PON2 antibody [EPR15295-82] was shown to specifically react with PON2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265927 (knockout cell lysate ab258128) was used. Wild-type and PON2 knockout samples were subjected to SDS-PAGE. ab183710 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON2 antibody [EPR15295-82] (ab183710)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling PON2 with ab183710 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON2 antibody [EPR15295-82] (ab183710)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling PON2 with ab183710 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Western blot - Anti-PON2 antibody [EPR15295-82] (ab183710)

All lanes : Anti-PON2 antibody [EPR15295-82] (ab183710) at 1/20000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : U87-MG cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 39 kDa
Observed band size: 39,41 kDa why is the actual band size different from the predicted?
Immunoprecipitation - Anti-PON2 antibody [EPR15295-82] (ab183710)

Western blot analysis of PON2 in HepG2 cell lysate immunoprecipitated with ab183710 at 1/50 dilution (Lane 1). Lane 2: TBS instead of HepG2 lysates.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Anti-PON2 antibody [EPR15295-82] (ab183710)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com