Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Mouse monoclonal [AF3E6] to PON2
Suitable for: Flow Cyt, WB, IP
Reacts with: Mouse, Rat, Human, Recombinant fragment
Isotype: IgG

Product name

Anti-PON2 antibody [AF3E6]
See all PON2 primary antibodies
Description

Mouse monoclonal [AF3E6] to PON2
Host species

Mouse
Specificity

We do not recommend to use this antibody in mouse and rat as very weak staining is observed.

Tested Applications & Species

Application	Species
Flow Cyt	Human
IP	Human
WB	Human Recombinant fragment

See all applications and species data

Immunogen

His tagged recombinant Human PON2 protein purified from E.coli
Positive control
- HeLa, HepG2 and 293T cell lysate.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

Preservative: 0.03% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, HEPES, 0.01% BSA
Concentration information loading...
Purity

Ammonium Sulphate Precipitation
Purification notes

Purified from TCS
Clonality

Monoclonal
Clone number

AF3E6
Isotype

IgG
Light chain type

kappa
Research areas

Western blot - Anti-PON2 antibody [AF3E6] (ab85340)

All lanes : Anti-PON2 antibody [AF3E6] (ab85340) at 1/1000 dilution

Lane 1 : HeLa Cell lysate
Lane 2 : HepG2 Cell lysate
Lane 3 : C6 Cell lysate

Predicted band size: 31 kDa

Ladder M.W(kDa):17, 25, 30, 46, 58, 80, 175
Flow Cytometry - Anti-PON2 antibody [AF3E6] (ab85340)

Overlay histogram showing HepG2 cells stained with ab85340 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85340, 0.1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG ( 1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Western blot - Anti-PON2 antibody [AF3E6] (ab85340)

All lanes : Anti-PON2 antibody [AF3E6] (ab85340) at 1/1000 dilution

Lane 1 : Paraoxonases 1 Recombinant Protein
Lane 2 : Paraoxonases 2 Recombinant Protein
Lane 3 : Paraoxonases 3 Recombinant Protein

Predicted band size: 31 kDa

Ladder M.W(kDa): 17, 25, 30, 46, 58, 80, 175
Immunoprecipitation - Anti-PON2 antibody [AF3E6] (ab85340)

Ladder M.W(kDa): 17, 25, 30, 46, 58, 80, 175

All lanes : Anti-PON2 antibody [AF3E6] (ab85340) at 1/1000 dilution

Lane 1 : HepG2 Cell Lysate 200ug
Lane 2 : Precipitated from HepG2 Cell Lysate 200ug using ab85340 2ug
Lane 3 : Precipitated from HepG2 Cell Lysate 200ug using ab85340 5ug
Lane 4 : Precipitated from PBS using ab85340 5ug
Western blot - Anti-PON2 antibody [AF3E6] (ab85340)

All lanes : Anti-PON2 antibody [AF3E6] (ab85340) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : 293T cell lysate

Predicted band size: 31 kDa
Observed band size: 41 kDa
why is the actual band size different from the predicted?
Western blot - Anti-PON2 antibody [AF3E6] (ab85340)

All lanes : Anti-PON2 antibody [AF3E6] (ab85340) at 1/1000 dilution

Lane 1 : PON1 Recombinant Protein
Lane 2 : PON2 Recombinant Protein
Lane 3 : PON3 Recombinant Protein
Lane 4 : HeLa Cell Lysate
Lane 5 : HepG2 Cell Lysate
Lane 6 : U87mg Cell Lysate

Predicted band size: 31 kDa
Observed band size: 31,41 kDa why is the actual band size different from the predicted?

Exposure time: 1 minute
Western blot - Anti-PON2 antibody [AF3E6] (ab85340)

All lanes : Anti-PON2 antibody [AF3E6] (ab85340) at 1/1000 dilution

Lane 1 : HeLa Cell Lysate
Lane 2 : PC12 Cell Lysate
Lane 3 : H9C2 Cell Lysate
Lane 4 : C6 Cell Lysate
Lane 5 : L929 Cell Lysate
Lane 6 : NIH3T3 Cell Lysate
Lane 7 : Rat Liver Lysate
Lane 8 : Mouse Liver Lysate

Secondary
All lanes : Mouse-specific secondary at 1/5000 dilution

Predicted band size: 31 kDa
Observed band size: 31,41 kDa why is the actual band size different from the predicted?

Exposure time: 5 minutes

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