Anti-SARS-CoV-2 Spike Glycoprotein RBD antibody [5G8] (ab277628)
![Anti-SARS-CoV-2 Spike Glycoprotein RBD antibody [5G8] (ab277628)](https://www.abcam.com/ps/products/277/ab277628/Images/ab277628-438735-AntiSpike-glycoprotein-antibody-5G8-ICC-HEK293-cells-transfected-with-pCINeoMod-containing-the-SARSCoV2bd-cDNA.JPG "Anti-SARS-CoV-2 Spike Glycoprotein RBD antibody [5G8] (ab277628)")
Key features and details
- Mouse monoclonal [5G8] to SARS-CoV-2 Spike Glycoprotein RBD
- Suitable for: WB, ICC
- Isotype: IgG1
Overview
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Product name
Anti-SARS-CoV-2 Spike Glycoprotein RBD antibody [5G8]
See all SARS-CoV-2 Spike Glycoprotein RBD primary antibodies -
Description
Mouse monoclonal [5G8] to SARS-CoV-2 Spike Glycoprotein RBD -
Host species
Mouse -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: SARS-CoV-2
Predicted to work with: Human coronavirus
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Immunogen
Recombinant fragment corresponding to Human coronavirus SARS-CoV-2 Spike Glycoprotein RBD.
Database link: P0DTC2 -
Positive control
- WB: HEK-293 cells transfected with pCI-Neo-Mod containing the SARS-CoV2-bd cDNA, cells transfected with pCI-Neo-GFP vector expression construct containing containing the SARS-CoV2-bd cDNA. ICC: HEK-293 cells transfected with pCI-Neo-Mod vector including SARS-CoV2-bd cDNA.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.03% Sodium azide
Constituents: 49.07% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from Tissue Culture supernatant. -
Clonality
Monoclonal -
Clone number
5G8 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry - Anti-Spike glycoprotein antibody [5G8] (ab277628)
HEK-293 cells transfected with pCI-Neo-Mod vector including SARS-CoV2-bd cDNA stained for Spike glycoprotein using ab277628 (Red) at 1/1000 dilution in Immunocytochemistry. The nuclei of transfected and untransfected cells are shown in blue with Hoechst staining.
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![Western blot - Anti-Spike glycoprotein antibody [5G8] (ab277628)](https://www.abcam.com/ps/products/277/ab277628/Images/ab277628-438734-AntiSpike-glycoprotein-antibody-5G8-WB-HEK293-cells-transfected-with-pCINeoMod-and-HEK293-cells-transfected-with-pCINeoGFP-containing-the-SARSCoV2bd-cDNA.JPG)
Western blot - Anti-Spike glycoprotein antibody [5G8] (ab277628)Lanes 2-4 & 6-7 : Anti-SARS-CoV-2 Spike Glycoprotein RBD antibody [5G8] (ab277628) at 1/3000 dilution
Lanes 1 & 5 : Molecular weight ladder
Lane 2 : Non-transfected HEK-293 cells
Lane 3 : HEK-293 cells transfected with pCI-Neo-Mod containing the SARS-CoV2-bd cDNA
Lane 4 : HEK-293 cells transfected with pCI-Neo-GFP vector expression construct containing the SARS-CoV2-bd cDNA
Lane 6 : Full length recombinant SARS-CoV2 S-protein expressed in HEK293 cells at 1 µg
Lane 7 : Full length recombinant SARS-CoV2 S-protein expressed in HEK293 cells at 0.1 µg
Additional bands at: 60 kDa (possible Loading Control)The blot was also stained with rabbit polyclonal control antibody to HSP60 (red) at 1/5000 dilution revealing a single band at 60kDa in both transfected and non-transfected cells.
