Cytochrome c Apoptosis WB Antibody Cocktail (ab110415)
Key features and details
- Assay type: Quantitative
Overview
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Product name
Cytochrome c Apoptosis WB Antibody Cocktail
See all ApoTrack™ Cytochrome c Apoptosis kits -
Assay type
Quantitative -
Species reactivity
Reacts with: Human -
Product overview
The permeabilization of mitochondrial outer membrane and the subsequent release of cytochrome c and other apoptogenic proteins from mitochondrial intermembrane space into the cytoplasm is considered a hallmark of many apoptotic pathways. Therefore assaying these proteins in mitochondrial and cytoplasmic fractions is of prime interest for many researchers.
ab 110415 (MSA12) is a Western blot antibody cocktail that allows for the detection of cytochrome c in cytoplasmic and mitochondria-containing fractions for determining the proportion of released cytochrome c from mitochondria to the cytoplasm from apoptosis. The kit includes antibodies against a cytoplasmic protein marker, glyceraldehyde-3-phosphodehydrogenase (GAPDH), and 2 mitochondrial markers, pyruvate dehydrogenase subunit E1-alpha (a matrix marker), and ATP synthase subunit alpha (an inner membrane marker). This set of control markers allows for the monitoring and/or optimization of the permeabilization conditions.
Cocktail Antibodies:
Mouse anti Cyt. c monoclonal:
Amount: 50 µg
Working concentration: 1 µg/mlMouse anti GAPDH monclonal:
Amount: 5ug
Working concentration: 0.1 µg/mlMouse anti PDH-E1-alpha monoclonal:
Amount: 100ug
Working concentration: 2 µg/mlMouse anti C-V-alpha monoclonal:
Amount: 25ug
Working concentration: 0.5 µg/ml -
Notes
This product was previously called ApoTrack™ Cytochrome c Apoptosis WB Antibody Cocktail
Other apoptosis assays
For more apoptosis assays, review the apoptosis assay and apoptosis marker guide.
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Tested applications
Suitable for: WBmore details
Properties
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Storage instructions
Please refer to protocols. -
Components 180 µg HeLa Lysate Control 1 x 50µg Pre-mixed Solution of 4 monoclonal Antibodies 1 x 180µg -
Research areas
Images
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Western blot - ApoTrack™ Cytochrome c Apoptosis WB Antibody Cocktail (ab110415)In this experiment, apoptosis was induced in Jurkat and 143B osteosarcoma cells by FAS and also by treatment with staurosporine (HeLa cells were also treated, but only with STS). Mitochondrial and cytoplasmic fractions were isolated (using kit Cell Fractionation Kit ab109719/MS861) and probed using ab110415 (MSA12). As is clear from the gels, cytochrome c has translocated partially in FAS-induced cells and STS-treated osteosarcoma cells, and almost completely in STS-treated Jurkat and HeLa cells. The three control targets allow for verification of the "cleanness" of the cell fractionation.
