Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)
![Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)](https://www.abcam.com/ps/products/192/ab192617/Images/ab192617-230473-anti-dap13-antibody-epr15867-28-western-blot.jpg "Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15867-28] to DAP13/NDUFA12
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-DAP13/NDUFA12 antibody [EPR15867-28]
See all DAP13/NDUFA12 primary antibodies -
Description
Rabbit monoclonal [EPR15867-28] to DAP13/NDUFA12 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P RatHumanWB MouseRatHuman
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa cells; Human fetal heart and skeletal muscle tissue lysates; Mouse brain, heart and kidney tissue lysates; Rat muscle tissue lysate; Human brain and stomach tissues; Rat kidney tissue.
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General notes
This product was previously labelled as DAP13
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15867-28 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)All lanes : Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617) at 1/1000 dilution
Lane 1 : mouse brain tissue lysate
Lanes 2 & 4 : mouse heart tissue lysate
Lanes 3 & 5 : mouse kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 17 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)](https://www.abcam.com/ps/products/192/ab192617/Images/ab192617-230467-anti-dap13-antibody-epr15867-28-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)Immunofluorescent analysis of HeLa cells (100% Methanol-fixed, 0.1% triton X-100-permealized) labeling DAP13/NDUFA12 with ab192617 at 1/100 dilution (20 μg/ml). A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (ab150077) was used as secondary antibody. Cells were fixed with Counterstain: DAPI.
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![Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)](https://www.abcam.com/ps/products/192/ab192617/Images/ab192617-230474-anti-dap13-antibody-epr15867-28-western-blot.jpg)
Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617) at 1/5000 dilution + Rat muscle tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 17 kDa
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![Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)](https://www.abcam.com/ps/products/192/ab192617/Images/ab192617-230472-anti-dap13-antibody-epr15867-28-western-blot.jpg)
Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617) at 1/5000 dilution + Human fetal heart tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 17 kDa
-
![Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)](https://www.abcam.com/ps/products/192/ab192617/Images/ab192617-230471-anti-dap13-antibody-epr15867-28-western-blot.jpg)
Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)All lanes : Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617) at 1/1000 dilution
Lane 1 : Human fetal heart tissue lysate
Lane 2 : Human skeletal muscle tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 17 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)](https://www.abcam.com/ps/products/192/ab192617/Images/ab192617-230470-anti-dap13-antibody-epr15867-28-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)Immunohistochemical analysis of paraffin-embedded Rat muscle tissue labeling DAP13/NDUFA12 with ab192617 at 1/1000 dilution (2μg/ml). A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)](https://www.abcam.com/ps/products/192/ab192617/Images/ab192617-230469-anti-dap13-antibody-epr15867-28-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling DAP13/NDUFA12 with ab192617 at 1/1000 dilution (2μg/ml). A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)](https://www.abcam.com/ps/products/192/ab192617/Images/ab192617-230468-anti-dap13-antibody-epr15867-28-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling DAP13/NDUFA12 with ab192617 at 1/1000 dilution (2μg/ml). A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)](https://www.abcam.com/ps/products/192/ab192617/Images/ab192617-9-benefits-of-recombinant-antibodies.png)
Anti-DAP13/NDUFA12 antibody [EPR15867-28] (ab192617)
