Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 02, 2021

Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15867] to DAP13/NDUFA12
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-DAP13/NDUFA12 antibody [EPR15867]
See all DAP13/NDUFA12 primary antibodies
Description

Rabbit monoclonal [EPR15867] to DAP13/NDUFA12
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Mouse Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human fetal heart, fetal skeletal muscle, fetal kidney, fetal liver lysates. Mouse brain, rat brain, rat muscle lysates. Human pancreas tissue, mouse liver tissue. Hela cells.
General notes
This product was previously labelled as DAP13

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15867
Isotype

IgG
Research areas

Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607)

All lanes : Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607) at 1/1000 dilution

Lane 1 : Human fetal heart
Lane 2 : Human skeletal muscle

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 17,7 kDa
Immunocytochemistry/ Immunofluorescence - Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607)

Immunofluorescent analysis of Hela cells permeabilised in 0.1% Triton X-100 and fixed in 4% paraformaldehyde labeling DAP13/NDUFA12 with ab192607 at 1/50 (5.9 μg/ml) and Goat anti rabbit IgG(Alexa Fluor® 488) ab150077. Counterstained with DAPI.
Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607)

All lanes : Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607) at 1/5000 dilution

Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal liver lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 17,7 kDa
Additional bands at: 7 kDa (possible isoform)
Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607)

Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607) at 1/5000 dilution + Rat muscle at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 17,7 kDa
Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607)

All lanes : Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607) at 1/1000 dilution

Lane 1 : Mouse brain
Lane 2 : Rat brain

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 17,7 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607)

Immunohistochemical analysis of formalin fixed paraffin embedded Human pancreas labeling DAP13/NDUFA12 with ab192607 at 1/600 (0.5μg/ml) dilution and prediluted HRP polymer to Rabbit/Mouse IgG as secondary.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607)

Immunohistochemical analysis of formalin fixed paraffin embedded mouse liver labeling DAP13/NDUFA12 with ab192607 at 1/600 (0.5μg/ml) dilution and prediluted HRP polymer to Rabbit/Mouse IgG as secondary.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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