Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15867] to DAP13/NDUFA12
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-DAP13/NDUFA12 antibody [EPR15867]
    See all DAP13/NDUFA12 primary antibodies

  • Description

    Rabbit monoclonal [EPR15867] to DAP13/NDUFA12

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal heart, fetal skeletal muscle, fetal kidney, fetal liver lysates. Mouse brain, rat brain, rat muscle lysates. Human pancreas tissue, mouse liver tissue. Hela cells.

  • General notes

     

     

     This product was previously labelled as DAP13

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607)
    Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607)
    All lanes : Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607) at 1/1000 dilution

    Lane 1 : Human fetal heart
    Lane 2 : Human skeletal muscle

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 17,7 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607)
    Immunocytochemistry/ Immunofluorescence - Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607)

    Immunofluorescent analysis of Hela cells permeabilised in 0.1% Triton X-100 and fixed in 4% paraformaldehyde labeling DAP13/NDUFA12 with ab192607 at 1/50 (5.9 μg/ml) and Goat anti rabbit IgG(Alexa Fluor® 488) ab150077. Counterstained with DAPI.

  • Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607)
    Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607)
    All lanes : Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607) at 1/5000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 17,7 kDa
    Additional bands at: 7 kDa (possible isoform)

  • Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607)
    Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607)
    Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607) at 1/5000 dilution + Rat muscle at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 17,7 kDa

  • Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607)
    Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607)
    All lanes : Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607) at 1/1000 dilution

    Lane 1 : Mouse brain
    Lane 2 : Rat brain

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 17,7 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607)

    Immunohistochemical analysis of formalin fixed paraffin embedded Human pancreas labeling DAP13/NDUFA12 with ab192607 at 1/600 (0.5μg/ml) dilution and prediluted HRP polymer to Rabbit/Mouse IgG as secondary.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607)

    Immunohistochemical analysis of formalin fixed paraffin embedded mouse liver labeling DAP13/NDUFA12 with ab192607 at 1/600 (0.5μg/ml) dilution and prediluted HRP polymer to Rabbit/Mouse IgG as secondary.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607)
    Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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