Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ORP1 antibody [EPR8646] - BSA and Azide free (ab248366)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8646] to ORP1 - BSA and Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ORP1 antibody [EPR8646] - BSA and Azide free
    See all ORP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR8646] to ORP1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A549, 293T, U87-MG cell lysates; Mouse brain and heart lysates; Rat brain lysates Flow Cyt: A549 Cells ICC/Flow Cyt: A549 and U87-MG cells

  • General notes

    Ab248366 is the carrier-free version of ab131165. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248366 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR8646

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-ORP1 antibody [EPR8646] - BSA and Azide free (ab248366)
    Flow Cytometry - Anti-ORP1 antibody [EPR8646] - BSA and Azide free (ab248366)
    Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling ORP1 with purified ab131165 at 1/60 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131165)
  • Immunocytochemistry/ Immunofluorescence - Anti-ORP1 antibody [EPR8646] - BSA and Azide free (ab248366)
    Immunocytochemistry/ Immunofluorescence - Anti-ORP1 antibody [EPR8646] - BSA and Azide free (ab248366)
    Immunocytochemistry/ Immunofluorescence analysis of A549 (Human lung carcinoma epithelial cell) cells labeling ORP1 with purified ab131165 at 1:200 dilution (2.8 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (anti orp1 antibody epr8646 immunocytochemistry a549 human)
  • Anti-ORP1 antibody [EPR8646] - BSA and Azide free (ab248366)
    Anti-ORP1 antibody [EPR8646] - BSA and Azide free (ab248366)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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