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Microbiology Organism Virus RNA Virus ssRNA positive strand virus SARS Coronavirus

Recombinant Human coronavirus SARS-COV-2 Nsp7 protein (His tag) (ab277124)

Recombinant Human coronavirus SARS-COV-2 Nsp7 protein (His tag) (ab277124)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Tags: His tag N-Terminus
  • Suitable for: SDS-PAGE

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Description

  • Product name

    Recombinant Human coronavirus SARS-COV-2 Nsp7 protein (His tag)
  • Purity

    > 95 % SDS-PAGE.
    >99% pure by HP-SEC.
  • Endotoxin level

  • Expression system

    Escherichia coli
  • Accession

    YP_009725303.1
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human coronavirus
    • Sequence

      SKMSDVKCTS VVLLSVLQQL RVESSSKLWA QCVQLHNDIL LAKDTTEAFE KMVSLLSVLL SMQGAVDINK LCEEMLDNRA TLQ
    • Predicted molecular weight

      11 kDa including tags
    • Molecular weight information

      The protein has a calculated MW of 11.3 kDa. The protein migrates as 12 kDa under reducing (R) condition (SDS-PAGE). 23.17 kDa verified by MALS.
    • Amino acids

      1 to 83
    • Tags

      His tag N-Terminus
    • Additional sequence information

      10X His tag. YP_009725303.1
  • Associated products

    • Related Products

      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-6X His tag® antibody [4D11] (ab5000)
      • Anti-6X His tag® antibody (ab9108)

    Specifications

    Our Abpromise guarantee covers the use of ab277124 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Liquid
    • Additional notes

      Recommended to give a quick spin to recover the volume before opening the cap.

      Avoid vigorous shaking or vortexing.

      To avoid surface adsorption and inactivation, the protein must NOT be aliquoted to

      Under the following conditions carrier protein (0.1% w/v ) HSA or BSA MUST be included in the protein buffer:

      1. Aliquots of

      2. The protein is to be diluted below the stock concentration

      3. Long term storage

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

      pH: 7.40
      Constituents: 80% PBS, 20% Glycerol (glycerin, glycerine)

      0.2 µm filtered solution

Images

  • SDS-PAGE - Recombinant SARS Nsp7 protein (ab277124)
    SDS-PAGE - Recombinant SARS Nsp7 protein (ab277124)

    SDS-PAGE of ab277124.

    The protein has a calculated MW of 11.3 kDa. The protein migrates as 12 kDa under reducing (R) condition (SDS-PAGE).

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab277124? Please let us know so that we can cite the reference in this datasheet.

    ab277124 has not yet been referenced specifically in any publications.

    Preparation and Storage

    • Stability and Storage

      Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

      pH: 7.40
      Constituents: 80% PBS, 20% Glycerol (glycerin, glycerine)

      0.2 µm filtered solution

    Images

    • SDS-PAGE - Recombinant SARS Nsp7 protein (ab277124)
      SDS-PAGE - Recombinant SARS Nsp7 protein (ab277124)

      SDS-PAGE of ab277124.

      The protein has a calculated MW of 11.3 kDa. The protein migrates as 12 kDa under reducing (R) condition (SDS-PAGE).

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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