Recombinant human coronavirus SARS-CoV-2 PLpro protein (Active) (ab277615)
Key features and details
- Expression system: Escherichia coli
- Purity: > 80% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human coronavirus SARS-CoV-2 PLpro protein (Active)
See all SARS-CoV-2 PLpro proteins and peptides -
Biological activity
The protease specific activity of ab277615 was ≥450 pmol/min/mg at an enzyme concentration of 4 µg as per proteolytic activity assay protocol.
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Purity
> 80 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
Yes -
Nature
Recombinant -
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Species
Human coronavirus -
Sequence
EVRTIKVFTT VDNINLHTQV VDMSMTYGQQ FGPTYLDGAD VTKIKPHNSH EGKTFYVLPN DDTLRVEAFE YYHTTDPSFL GRYMSALNHT KKWKYPQVNG LTSIKWADNN CYLATALLTL QQIELKFNPP ALQDAYYRAR AGEAANFCAL ILAYCNKTVG ELGDVRETMS YLFQHANLDS CKRVLNVVCK TCGQQQTTLK GVEAVMYMGT LSYEQFKKGV QIPCTCGKQA TKYLVQQESP FVMMSAPPAQ YELKHGTFTC ASEYTGNYQC GHYKHITSKE TLYCIDGALL TKSSEYKGPI TDVFYKENSY TTTIKPVTY -
Molecular weight information
38 kDa by SDS-PAGE -
Amino acids
1564 to 1882 -
Tags
His tag N-Terminus -
Additional sequence information
The gene accession number is QHD43415.
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Images
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Functional Studies - Recombinant human coronavirus SARS-CoV-2 PLpro protein (ab277615)
The protease specific activity of ab277615 was ≥450 pmol/min/mg at an enzyme concentration of 4 µg in a proteolytic activity assay using synthetic FRET peptide as substrate specific for PLpro.
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Functional Studies - Recombinant human coronavirus SARS-CoV-2 PLpro protein (ab277615)The deubiquitinase specific activity of ab277615 was 7.2 nmol/min/mg in a deubiquitinase activity assay protocol using recombinant human ubiquitin-based proluciferin as substrate. The IC50 of the reversible deubiquitinase inhibitor PR-619 was 10.13 µM in the PLpro inhibition profile assay.
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SDS-PAGE - Recombinant human coronavirus SARS-CoV-2 PLpro protein (ab277615)SDS-PAGE analysis of ab277615.